Currents through Hv1 channels deplete protons in their vicinity.

The Journal of General Physiology
Víctor De-la-RosaLeón D Islas

Abstract

Proton channels have evolved to provide a pH regulatory mechanism, affording the extrusion of protons from the cytoplasm at all membrane potentials. Previous evidence has suggested that channel-mediated acid extrusion could significantly change the local concentration of protons in the vicinity of the channel. In this work, we directly measure the proton depletion caused by activation of Hv1 proton channels using patch-clamp fluorometry recordings from channels labeled with the Venus fluorescent protein at intracellular domains. The fluorescence of the Venus protein is very sensitive to pH, thus behaving as a genetically encoded sensor of local pH. Eliciting outward proton currents increases the fluorescence intensity of Venus. This dequenching is related to the magnitude of the current and not to channel gating and is dependent on the pH gradient. Our results provide direct evidence of local proton depletion caused by flux through the proton-selective channel.

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Citations

Oct 4, 2018·American Journal of Physiology. Cell Physiology·Clarisse FusterBruno Allard
Jan 15, 2020·Journal of Cell Science·Arturo Matamoros-Volante, Claudia L Treviño
May 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Lea WobigThomas K Berger

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