DOI: 10.1101/545574Feb 10, 2019Paper

Cusp-artifacts in high order superresolution optical fluctuation imaging

BioRxiv : the Preprint Server for Biology
Xiyu Yi, Shimon Weiss

Abstract

Superresolution optical fluctuation imaging (SOFI) is a simple and affordable super-resolution imaging technique and attracted a growing community over the past decade. However, the theoretical resolution enhancement of high order SOFI is still not fulfilled. In this study, we identify "cusp artifacts" in high order SOFI images, and show that the high-order cumulants, odd-order moments and balanced-cumulants (bSOFI) are highly vulnerable to cusp artifacts. Our study provides guidelines for developing and screening for fluorescence probes and improving data acquisition for SOFI. The new insight is important to inspire positive utilization of the cusp artifacts.

Related Concepts

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.