Cutaneous cell and extracellular matrix responses to ultraviolet-B irradiation

Journal of Cellular Physiology
I PâquetMahmoud Rouabhia

Abstract

The present study examined fibroblasts and keratinocytes in monolayers and cultured within dermal and skin substitutes and their use in assessing the effect of UVB irradiation on cutaneous cells and extracellular matrix organization. Dermal substitutes (DS) were produced by incorporating normal fibroblasts into a collagen lattice and skin substitutes (SS) were obtained by seeding normal keratinocytes onto the DS. Keratinocyte monolayers, fibroblast monolayers, DS, and SS were exposed once a day to a UVB source (10 mJ/cm2). The irradiation protocol was stopped when the keratinocytes of the non-irradiated cultures (control groups) had reached confluence. Microscopic observations revealed that UVB radiation decreased both fibroblast and keratinocyte growth and enhanced their differentiation resulting in (1) less fibroblasts in the DS and SS, and (2) incomplete coverage of the DS by keratinocytes. Microscopic observations and histological analyses revealed major morphological changes. Both cell types became bigger and presented wide nuclei and vacuoles in the cytoplasm. No organized deep epidermal layer was observed in irradiated compared to non-irradiated SS. Irradiated DS and SS extracellular matrices showed an irregular aggregat...Continue Reading

References

Oct 1, 1992·The Journal of Investigative Dermatology·M J PetersenS Craig
Oct 1, 1992·The British Journal of Dermatology·C A López ValleL Germain
Nov 1, 1991·The Journal of Investigative Dermatology·M YamauchiD T Woodley
Oct 1, 1990·Photochemistry and Photobiology·G KelfkensJ C van der Leun
Feb 1, 1990·Journal of Cellular Physiology·P S FriedmannJ N Matthews
Dec 1, 1988·Cancer Metastasis Reviews·J Longstreth
Feb 1, 1985·The Journal of Investigative Dermatology·R S SternH L Bleich
Jul 1, 1970·International Journal of Dermatology·R G FreemanM A Carnes
Jan 1, 1984·Annual Review of Biochemistry·D R EyreP M Gallop
Jan 1, 1981·Advances in Cancer Research·M L Kripke
May 1, 1982·The Journal of Investigative Dermatology·I M Braverman, E Fonferko
Jan 1, 1993·The British Journal of Dermatology·T HorioY Asada
Jun 1, 1993·The Journal of Investigative Dermatology·A A VinkR A Baan
Aug 1, 1993·The Journal of Investigative Dermatology·M WlaschekK Scharffetter-Kochanek
Nov 6, 1993·Lancet·A Jeevan, M L Kripke
May 1, 1993·Photochemistry and Photobiology·D Nelson, R J Gay
Aug 1, 1993·The Journal of Investigative Dermatology·J W GilmourM Norval
Oct 1, 1993·Journal of Cellular Physiology·N S DissanayakeR S Mason
Oct 1, 1993·The Journal of Investigative Dermatology·J E SavageR Anderson
Jun 1, 1995·In Vitro Cellular & Developmental Biology. Animal·F A AugerL Germain
Oct 1, 1962·The Journal of Investigative Dermatology·J G SMITHR D CLARK

❮ Previous
Next ❯

Citations

Aug 24, 1999·Journal of Cellular Physiology·N Chouinard, M Rouabhia
Jun 15, 1999·Medical & Biological Engineering & Computing·O DamourA F Black
Oct 20, 2001·Journal of Dermatological Science·Y W RyooK S Lee
Feb 16, 2000·Journal of Dermatological Science·M MatsuiA Aioi
Jan 24, 2019·International Journal of Environmental Research and Public Health·Humidah AlanaziMahmoud Rouabhia
Apr 28, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·C LuitaudM Rouabhia
Apr 2, 2019·Journal of Cosmetic Dermatology·Annabelle St-PierreIsabel Desgagné-Penix
Mar 16, 2004·Experimental Dermatology·H K BiesalskiT Schwarz
Aug 15, 1998·Biochemical and Biophysical Research Communications·C HuardJ P Tremblay

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Burns : Journal of the International Society for Burn Injuries
M L Stoner, F M Wood
Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Mahmoud Rouabhia, Noëlla Deslauriers
Toxicology in Vitro : an International Journal Published in Association with BIBRA
H B Koëter
© 2021 Meta ULC. All rights reserved