Cutaneous optical coherence tomography for longitudinal volumetric assessment of intradermal volumes in a mouse model.

Scientific Reports
Kornelia SchuetzenbergerRené M Werkmeister

Abstract

Clinical evaluation of skin lesions requires precise and reproducible technologies for their qualitative and quantitative assessment. In this study, we investigate the applicability of a custom-built dermatologic OCT system for longitudinal assessment of intradermal volumes in a mouse model. The OCT, based on an akinetic swept laser working at 1310 nm was employed for visualization and quantification of intradermal deposits of three different hyaluronic acid-based hydrogel formulations - one commercial and two test substances. Hydrogels were applied in 22 BALB/c mice, and measurements were performed over a six-month time period. All hydrogels increased in volume within the first weeks and degraded steadily thereafter. The half-lifes of the test hydrogels (27.2 ± 13.6 weeks for Hydrogel 1, 31.5 ± 17.2 weeks for Hydrogel 2) were higher in comparison to the commercially available HA hydrogel (21.4 ± 12.0 weeks), although differences were not significant. The sphericity parameter was used for evaluation of the deposit geometry. While on the injection day the sphericities were similar (~0.75 ± 0.04), at later time points significant differences between the different test substances were found (T24: PRV 0.59 ± 0.09, Hydrogel 1 0.70 ±...Continue Reading

References

Apr 14, 2007·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Jesse M OlmedoDavid L Swanson
Mar 6, 2008·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Eric S SchweigerDaniel J Aires
Jan 25, 2011·Ultrasound in Medicine & Biology·Carmel M MoranHamish M Fraser
Feb 18, 2012·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Alexander T HillelJennifer H Elisseeff
Mar 16, 2012·Facial Plastic Surgery : FPS·Timothy M GrecoSeth A Yellin
Jan 15, 2013·Journal of Biomedical Optics·Elke SattlerJulia Welzel
Jan 23, 2013·Journal of Biomedical Optics·Martina Ulrich, Susanne Lange-Asschenfeldt
Jul 31, 2014·Journal of Innovative Optical Health Sciences·Elijah YewPeter T C So
Aug 15, 2014·Optics Letters·Yong ZhouLihong V Wang
Sep 17, 2014·Archives of Dermatological Research·Alia Arif HussainGregor Borut Ernst Jemec
Sep 23, 2014·Journal of Biophotonics·Thilo GambichlerTanja Maier
Oct 21, 2015·Journal of the American Academy of Dermatology·Jared JagdeoAlastair Carruthers
Oct 23, 2015·Clinical, Cosmetic and Investigational Dermatology·Uwe Wollina, Alberto Goldman
Feb 13, 2016·AJR. American Journal of Roentgenology·Carolina M Botar-JidSorin M Dudea
Feb 14, 2016·European Journal of Medical Research·Bettina Alexandra BuhrenPeter Arne Gerber
Apr 14, 2016·Aesthetic Surgery Journal
Jul 22, 2016·Anais Brasileiros De Dermatologia·Elisa de Oliveira BarcauiCarlos Baptista Barcaui
Oct 24, 2017·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·N De CarvalhoJ Welzel
Apr 28, 2018·Journal of Biomedical Optics·Jonas OlsenGregor B Jemec
Jul 12, 2018·Journal of Biomedical Optics·Zahra TuraniMohammad Reza Nasiri Avanaki
Dec 13, 2018·Lasers in Medical Science·Ali Rajabi-EstarabadiKeyvan Nouri
Jan 9, 2019·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Feb 9, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Mengyang Liu, Wolfgang Drexler
Mar 21, 2019·Biomedical Optics Express·Martin PfisterRené M Werkmeister
Aug 15, 2019·Pharmaceutics·Sonia TrombinoRoberta Cassano
Aug 20, 2019·Advanced Drug Delivery Reviews·Alberto Passi, Davide Vigetti

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Methods Mentioned

BETA
imaging technique
light microscopy

Software Mentioned

ARRIVE
view2
Fiji
HFUS
OCT
SPSS Statistics
Fiji4
SPSS

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