Cuticular proteins from the lobster, Homarus americanus

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
M KraghS O Andersen

Abstract

The urea-extractable proteins from calcified regions of intermoult cuticle of the lobster, Homarus americanus, have been separated by two-dimensional electrophoresis, showing that the extracts contain a large number of proteins. The major proteins have isoelectric points between 4 and 9, and their apparent molecular weights are between 5 and 30 kDa. Two of the proteins have been purified by a combination of ion-exchange chromatography, gel-filtration and RP-HPLC, and their complete amino acid sequences were determined by a combination of mass spectrometry and automated Edman degradation. Although they were purified from a single animal, both proteins were obtained as two isoforms. The isoforms of the smaller protein (HaCP4.6) differed only in a single position (phenylalanine/isoleucine), and the isoforms of the larger protein (HaCP11.6) differed in two positions (valine/isoleucine and glutamine/lysine). HaCP11.6 is N-terminally blocked by a pyroglutamate residue. Variants of an 18-residue motif are a characteristic feature of both sequences: it occurs twice in HaCP4.6 and four times in HaCP11.6. Comparison of the sequence to sequences published for cuticular proteins from other arthropods shows that the repeated motif is also p...Continue Reading

References

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Feb 1, 1997·Insect Biochemistry and Molecular Biology·S O AndersenP Roepstorff

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Citations

Jun 9, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Anna Wynn, Thomas H Shafer
Apr 23, 2003·Biochemical and Biophysical Research Communications·Isao FukudaToshiki Watanabe
Jun 7, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T WatanabeM Kono
May 13, 1999·Insect Biochemistry and Molecular Biology·V A IconomidouS J Hamodrakas
Feb 27, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·S O Andersen
Jul 30, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·S O Andersen
Mar 16, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T IkeyaT Watanabe
Mar 12, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Nicholas DitzelPeter Højrup
Apr 8, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M NousiainenS O Andersen
May 17, 2014·Small·Lessa Kay GrunenfelderDavid Kisailus
Dec 26, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Lindsay M Faircloth, Thomas H Shafer

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