Cutting edge: single-chain trimers of MHC class I molecules form stable structures that potently stimulate antigen-specific T cells and B cells

The Journal of Immunology : Official Journal of the American Association of Immunologists
Yik Y L YuTed H Hansen

Abstract

We report in this work the expression and characterization of class I molecules expressed as single-chain trimers consisting of an antigenic peptide-spacer-beta(2)-microglobulin-spacer H chain. Our results indicate that these single-chain constructs assemble efficiently, maintain their covalent structure, and are unusually stable at the cell surface. Consequently, these constructs are at least 1000-fold less accessible to exogenous peptide than class I molecules loaded with endogenous peptides, and they are potent simulators of peptide-specific CTL and Abs. Our combined findings suggest that single-chain trimers may have applications as DNA vaccines against virus infection or tumors.

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M G MageD H Margulies
Jul 27, 1990·Cell·A TownsendA Tse
Mar 1, 1989·The Journal of Experimental Medicine·F R Carbone, M J Bevan
Jan 14, 1994·Cell·K A HogquistF R Carbone
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·K ToshitaniW F Bodmer
Oct 1, 1996·The Journal of Experimental Medicine·M CorrH Tighe
May 23, 1998·Annual Review of Immunology·E Pamer, P Cresswell
Jun 6, 1998·Trends in Microbiology·H HengelU H Koszinowski
Aug 23, 2000·Immunology Today·B SeligerS Ferrone
Nov 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·N B MyersT H Hansen
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D H ChungD H Margulies
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T M HornellJ M Connolly

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Citations

Apr 15, 2008·American Journal of Reproductive Immunology : AJRI·Joan K Riley, Wayne M Yokoyama
Oct 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yangbing ZhaoRichard A Morgan
Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Dibyendu SamantaSteven C Almo
Jul 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lijin LiWilliam E Gillanders
Jan 13, 2015·Protein Engineering, Design & Selection : PEDS·Toru HondaTomoko Yoshino
Apr 25, 2017·Nature Nanotechnology·Santiswarup SinghaPere Santamaria
Feb 1, 2019·Nature Methods·Alok V JoglekarDavid Baltimore
Oct 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Denis HudrisierHenri Vié
Sep 27, 2005·Immunological Reviews·Ted H HansenDaved H Fremont
Nov 15, 2006·Immunological Reviews·Wayne M Yokoyama, Sungjin Kim
Jul 2, 2009·Xenotransplantation·Ethel J Gordon, Vinaya Kelkar
Oct 8, 2009·International Journal of Cancer. Journal International Du Cancer·Ying WuBin Gao
Aug 14, 2013·The Journal of Experimental Medicine·John A H HoerterNicholas R J Gascoigne
Jun 14, 2012·Protein Engineering, Design & Selection : PEDS·Cheng-Pao ChenSteve R Roffler
Feb 7, 2019·Oncoimmunology·Nadia MensaliSébastien Wälchli
Mar 24, 2020·Nature Structural & Molecular Biology·Colin E CorrentiPhilip Bradley
Jun 15, 2007·European Journal of Immunology·Whitney E PurthaMichael S Diamond
May 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Steven M TruscottTed H Hansen
Nov 2, 2005·Cancer Immunology, Immunotherapy : CII·Ted H Hansen, Lonnie Lybarger
Dec 4, 2010·Antioxidants & Redox Signaling·Eleni KotsiouKeith G Gould
Feb 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dikla BerkoGideon Gross
Apr 6, 2011·Immunotherapy·Lindzy F DodsonPeter Goedegebuure
Jul 26, 2017·Frontiers in Immunology·Bing-Mae ChenSteve R Roffler
Dec 19, 2017·Cancer Immunology, Immunotherapy : CII·Else Marit InderbergSébastien Wälchli
Jan 11, 2018·Human Gene Therapy·Max A ChengT-C Wu
Jul 8, 2020·Nature Methods·Alok V Joglekar, Guideng Li
Apr 4, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael J PalmowskiKeith G Gould
Dec 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alon MargalitGideon Gross
May 11, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Beatriz M CarrenoGerald P Linette
May 16, 2008·Medicinal Research Reviews·Tian-Lu Cheng, Steve Roffler
Mar 10, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sojung KimTed H Hansen
Dec 28, 2012·Human Gene Therapy·Tae Heung KangT-C Wu

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