Cu/Zn superoxide dismutases in developing cotton fibers: evidence for an extracellular form.

Planta
Hee Jin KimBarbara A Triplett

Abstract

Hydrogen peroxide and other reactive oxygen species are important signaling molecules in diverse physiological processes. Previously, we discovered superoxide dismutase (SOD) activity in extracellular protein preparations from fiber-bearing cotton (Gossypium hirsutum L.) seeds. We show here, based on immunoreactivity, that the enzyme is a Cu/Zn-SOD (CSD). Immunogold localization shows that CSD localizes to secondary cell walls of developing cotton fibers. Five cotton CSD cDNAs were cloned from cotton fiber and classified into three subfamilies (Group 1: GhCSD1; Group 2: GhCSD2a and GhCSD2b; Group 3: GhCSD3 and GhCSD3s). Members of Group 1 and 2 are expressed throughout fiber development, but predominant during the elongation stage. Group 3 CSDs are also expressed throughout fiber development, but transiently increase in abundance at the transition period between cell elongation and secondary cell wall synthesis. Each of the three GhCSDs also has distinct patterns of expression in tissues other than fiber. Overexpression of cotton CSDs fused to green fluorescent protein in transgenic Arabidopsis demonstrated that GhCSD1 localizes to the cytosol, GhCSD2a localizes to plastids, and GhCSD3 is translocated to the cell wall. Subcellu...Continue Reading

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Citations

Oct 1, 2009·BMC Genomics·Pieter Bas KwakZhi Min Yang
Oct 9, 2014·Journal of Experimental Botany·Siân L RichardsJulia M Davies
Apr 28, 2012·Electrophoresis·Jowita Anna Dziedzic, Armando Gabriel McDonald
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Sep 22, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·J Vincent EdwardsDorne R Yager
Dec 21, 2021·Frontiers in Plant Science·Lisa S MydyRoland D Kersten

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Datasets Mentioned

BETA
CAA05633

Methods Mentioned

BETA
transgenic
PCR
gel filtration
gel
X-ray

Software Mentioned

Chlorop
GenomeWalker
ImageJ
ESLpred
MAFFT
WoLF PSORT
PLoc
Plant
Prism
SignalP

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