Cvt9/Gsa9 functions in sequestering selective cytosolic cargo destined for the vacuole

The Journal of Cell Biology
J KimDaniel J Klionsky

Abstract

Three overlapping pathways mediate the transport of cytoplasmic material to the vacuole in Saccharomyces cerevisiae. The cytoplasm to vacuole targeting (Cvt) pathway transports the vacuolar hydrolase, aminopeptidase I (API), whereas pexophagy mediates the delivery of excess peroxisomes for degradation. Both the Cvt and pexophagy pathways are selective processes that specifically recognize their cargo. In contrast, macroautophagy nonselectively transports bulk cytosol to the vacuole for recycling. Most of the import machinery characterized thus far is required for all three modes of transport. However, unique features of each pathway dictate the requirement for additional components that differentiate these pathways from one another, including at the step of specific cargo selection.We have identified Cvt9 and its Pichia pastoris counterpart Gsa9. In S. cerevisiae, Cvt9 is required for the selective delivery of precursor API (prAPI) to the vacuole by the Cvt pathway and the targeted degradation of peroxisomes by pexophagy. In P. pastoris, Gsa9 is required for glucose-induced pexophagy. Significantly, neither Cvt9 nor Gsa9 is required for starvation-induced nonselective transport of bulk cytoplasmic cargo by macroautophagy. The d...Continue Reading

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P BaumL Honig
Dec 1, 1985·Molecular and Cellular Biology·J M CreggK R Madden
May 5, 1995·Biochemical and Biophysical Research Communications·T NodaY Ohsumi
Mar 1, 1996·The Journal of Cell Biology·M N OdaD J Klionsky
Jul 26, 1996·The Journal of Biological Chemistry·T M HardingD J Klionsky
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·S V ScottD J Klionsky
Jan 1, 1996·Sub-cellular Biochemistry·G E MortimoreM Kadowaki
Jul 14, 1997·The Journal of Cell Biology·S V ScottD J Klionsky
Mar 28, 1998·The Journal of Biological Chemistry·T Noda, Y Ohsumi
Oct 6, 1998·Nature·N MizushimaY Ohsumi
May 8, 1999·Molecular Biology of the Cell·I TanidaE Kominami
Aug 5, 1999·Methods in Enzymology·S Labbé, D J Thiele
Sep 25, 1999·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·S VittoriniE Bergamini
Dec 28, 1999·Annual Review of Cell and Developmental Biology·D J Klionsky, Y Ohsumi
May 11, 2000·International Review of Cytology·J KimD J Klionsky
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·D F SealsA Price

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Citations

Feb 21, 2009·Histochemistry and Cell Biology·Sadaki Yokota, H Dariush Fahimi
Oct 18, 2002·Current Opinion in Cell Biology·Waheeda A Khalfan, Daniel J Klionsky
Jun 14, 2002·Trends in Cell Biology·Takeshi NodaYoshinori Ohsumi
Jun 19, 2012·Cell Death and Differentiation·Kuninori Suzuki
Oct 3, 2007·Nature Cell Biology·Zhiping Xie, Daniel J Klionsky
Jun 6, 2009·Nature Reviews. Molecular Cell Biology·Hitoshi NakatogawaYoshinori Ohsumi
Oct 26, 2005·Cell Death and Differentiation·T Yorimitsu, D J Klionsky
Feb 22, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroyuki MukaiyamaYasuyoshi Sakai
Apr 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Zhanna LipatovaNava Segev
Jul 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kai MaoDaniel J Klionsky
May 31, 2001·The Journal of Biological Chemistry·C W WangD J Klionsky
Feb 7, 2012·The Journal of Biological Chemistry·Noriko Kondo-OkamotoKoji Okamoto
Oct 5, 2002·The Journal of Biological Chemistry·Chao-Wen WangDaniel J Klionsky
May 13, 2004·The Journal of Biological Chemistry·Takahiro Shintani, Daniel J Klionsky
Sep 27, 2008·The Journal of Biological Chemistry·Tomotake Kanki, Daniel J Klionsky
Jan 5, 2011·Antioxidants & Redox Signaling·Tomotake KankiKoji Okamoto
Feb 18, 2003·Molecular Biology of the Cell·Hagai AbeliovichDaniel J Klionsky
May 20, 2005·Molecular Biology of the Cell·Heesun CheongDaniel J Klionsky
Jan 22, 2005·Molecular Biology of the Cell·Tomohiro Yorimitsu, Daniel J Klionsky
Dec 29, 2006·Molecular Biology of the Cell·Chiung-Ying Chang, Wei-Pang Huang
Sep 5, 2008·Molecular Biology of the Cell·Laura A SchroderWilliam A Dunn
Feb 22, 2008·Molecular Biology of the Cell·Tomoko KawamataYoshinori Ohsumi
Jul 17, 2009·Molecular Biology of the Cell·Taras Y NazarkoSuresh Subramani
Aug 9, 2013·Molecular Biology of the Cell·Zhanna LipatovaNava Segev
Aug 8, 2009·Biological Reviews of the Cambridge Philosophical Society·Iryna MonastyrskaFulvio Reggiori
Nov 22, 2005·Acta Pharmacologica Sinica·Ya-Ping YangZheng-Hong Qin
Nov 29, 2002·Eukaryotic Cell·Fulvio Reggiori, Daniel J Klionsky
Dec 10, 2009·Molecular and Cellular Biology·Yoshiaki KamadaYoshinori Ohsumi
Aug 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·H Abeliovich, D J Klionsky
Apr 7, 2012·International Journal of Cell Biology·Midori Umekawa, Daniel J Klionsky
Apr 27, 2012·International Journal of Cell Biology·Andreas TillSuresh Subramani

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Datasets Mentioned

BETA
AF309870

Methods Mentioned

BETA
PCR
immunoprecipitations
two-hybrid
Confocal Microscopy
electron microscopy
fluorescence microscopy
coimmunoprecipitation

Software Mentioned

BLAST
Adobe Photoshop

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