Cwp84, a Clostridium difficile cysteine protease, exhibits conformational flexibility in the absence of its propeptide

Acta Crystallographica. Section F, Structural Biology Communications
William J BradshawK Ravi Acharya

Abstract

In recent decades, the global healthcare problems caused by Clostridium difficile have increased at an alarming rate. A greater understanding of this antibiotic-resistant bacterium, particularly with respect to how it interacts with the host, is required for the development of novel strategies for fighting C. difficile infections. The surface layer (S-layer) of C. difficile is likely to be of significant importance to host-pathogen interactions. The mature S-layer is formed by a proteinaceous array consisting of multiple copies of a high-molecular-weight and a low-molecular-weight S-layer protein. These components result from the cleavage of SlpA by Cwp84, a cysteine protease. The structure of a truncated Cwp84 active-site mutant has recently been reported and the key features have been identified, providing the first structural insights into the role of Cwp84 in the formation of the S-layer. Here, two structures of Cwp84 after propeptide cleavage are presented and the three conformational changes that are observed are discussed. These changes result in a reconfiguration of the active site and exposure of the hydrophobic pocket.

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Citations

Nov 25, 2017·Journal of Cell Communication and Signaling·William J BradshawK Ravi Acharya
Oct 31, 2017·The FEBS Journal·William J BradshawK Ravi Acharya

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Methods Mentioned

BETA
protein array

Software Mentioned

Swiss
xia
CheckMyMetal
REFMAC
Coot
XDS
AIMLESS
Phaser
PdbViewer

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