CWR22 xenograft as an ex vivo human tumor model for prostate cancer gene therapy

Journal of the National Cancer Institute
L ChengN S Yang

Abstract

Lack of well-defined relevant in vivo or in vitro tumor models is one of the major limitations in assessing candidate therapeutic regimens, especially gene therapy, for prostate cancer. Since gene therapy is emerging as a potentially powerful therapeutic modality, it is desirable to evaluate this approach for the treatment of human prostate cancer. We sought to establish a relevant ex vivo tumor model for gene therapy studies of human prostate cancer. We constructed and established a transgenic human tumor model consisting of three major components: 1) human primary prostate cancer cells, CWR22, reactivated for growth after storage in liquid nitrogen; 2) a collagen gel ex vivo tissue culture system useful for short-term maintenance and manipulation of CWR22 cells under in vitro experimental conditions; and 3) a high-velocity, particle-mediated gene transfer system that is highly efficient in the ex vivo transfection of target cells. Prostate-specific antigen (PSA) levels in the cell culture media were monitored after transfecting CWR22 cells with candidate therapeutic genes, including the cytokines human interleukin 2 (IL-2) and granulocyte-macrophage colony-stimulating factor (GM-CSF), both as complementary DNAs [cDNAs]). CWR2...Continue Reading

Citations

Aug 26, 1999·In Vitro Cellular & Developmental Biology. Animal·R M SramkoskiJ W Jacobberger
Apr 1, 1997·British Journal of Urology·K Sikora, H Pandha
May 13, 1999·Progress in Histochemistry and Cytochemistry·L A CulpJ L Holleran
Aug 26, 2004·Advances in Cancer Research·Beatrice S Knudsen, Magnus Edlund
Apr 18, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J L HolleranL A Culp

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