CX3CL1/CX3CR1-signalling in the CD9/S100β/SOX2-positive adult pituitary stem/progenitor cells modulates differentiation into endothelial cells

Histochemistry and Cell Biology
Kotaro HoriguchiYukio Kato

Abstract

Approximately 8% of CD9-, S100β- and SOX2-triple positive (CD9/S100β/SOX2-positive) stem/progenitor cells in the anterior lobe of the rat pituitary gland have previously been shown to differentiate into endothelial cells in vitro, suggesting that they play a role in vascularisation as tissue-resident vascular precursor cells. In the present study, we focused on chemokine ligands to further characterise the CD9/S100β/SOX2-positive cells and found that they distinctively express CX3C chemokine ligand 1 (Cx3cl1). Immunohistochemical analysis of the anterior lobe showed that CX3CL1-positive cells comprised 7.8% in CD9-positive cells. By cultivation of the CD9-positive cells on laminin-coated plates, we observed that the expression levels of Cx3cl1 decreased, while those of Sox18, an endothelial cell-progenitor marker, and Cx3cr1, a CX3CL1 receptor, increased. Furthermore, in a rat model of prolactinoma, the most common pituitary tumour, which is accompanied by frequent neo-vasculogenesis in the anterior lobe, we have confirmed a decrease in Cx3cl1 expression and an increase in Cx3cr1 expression, as well as a prominent increase in Sox18 expression. These findings suggest that CX3CL1/CX3CR1 signalling in CD9/S100β/SOX2-positive cells...Continue Reading

References

Feb 1, 1986·American Journal of Clinical Pathology·M J VanstapelV D Desmet
Feb 13, 1997·Nature·J F BazanT J Schall
Apr 29, 1998·Nature·M Baggiolini
Sep 25, 1999·Archives of Histology and Cytology·K InoueS Ogawa
Dec 30, 1999·Journal of Leukocyte Biology·J K HarrisonL Belardinelli
Sep 14, 2001·Biochemical and Biophysical Research Communications·B M HoskingG E Muscat
Jul 9, 2002·Clinical and Experimental Immunology·S J ChakravortyC O S Savage
Apr 7, 2004·Journal of Atherosclerosis and Thrombosis·Tadaatsu ImaizumiKei Satoh
Mar 3, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Robert M StrieterJohn A Belperio
May 1, 2010·Nature Reviews. Drug Discovery·Sudarshan RajagopalRobert J Lefkowitz
Oct 30, 2016·Biochemical and Biophysical Research Communications·Kotaro HoriguchiYukio Kato
Jan 16, 2019·Stem Cells Translational Medicine·Gabriella Maria Fernandes-CunhaDavid Myung

❮ Previous
Next ❯

Citations

Jun 6, 2020·Histochemistry and Cell Biology·Douglas J Taatjes, Jürgen Roth
Nov 23, 2020·Histochemistry and Cell Biology·Kotaro HoriguchiShunji Ohsako

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Scanning electron microscopy
transfection

Software Mentioned

cellSens® Dimension

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

© 2021 Meta ULC. All rights reserved