DOI: 10.1101/19002394Sep 16, 2019Paper

CX3CR1 as a Respiratory Syncytial Virus Receptor in Pediatric Human Lung

MedRxiv : the Preprint Server for Health Sciences
Christopher S AndersonThomas J Mariani

Abstract

Objectives: Respiratory Syncytial Virus (RSV) is the most common cause of severe respiratory illness in young children. Data on the host factors that contribute to infection of young children by RSV are limited. The human chemokine receptor, CX3CR1, has recently been implicated as an RSV receptor in adult epithelial cells in vitro. Here we evaluate a role for CX3CR1 in pediatric lung RSV infections. Study Design: CX3CR1 transcript levels were assessed in pediatric airways using existing RNAseq data characterizing gene expression in the upper and lower airways. Tissue localization and cell specific expression of CX3CR1 in pediatric lung tissue was confirmed using in situ hybridization and immunohistochemistry. The role of CX3CR1 in RSV infection was also investigated in vitro using a novel, physiological model of pediatric epithelial cells. Results: Low levels of CX3CR1 transcript were often, but not always, expressed in both the upper (62%) and lower airways (36%) of pediatric subjects between 1 month and 8 years of age. CX3CR1 transcript and protein expression was detected in epithelial cells of intact, normal human pediatric lung tissues. In vitro, CX3CR1 expression was readily detected on primary cultures of differentiated p...Continue Reading

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