CX3CR1 delineates temporally and functionally distinct subsets of myeloid-derived suppressor cells in a mouse model of ovarian cancer

Immunology and Cell Biology
Kevin M HartBrent Berwin

Abstract

Expression of the chemokine receptor CX3CR1 has been used to identify distinct populations within the monocyte, macrophage and dendritic cell lineages. Recent evidence indicates that CX3CR1-positive subsets of myeloid cells play distinct and important roles in a wide range of immunological maladies, and thus the use of CX3CR1 expression has leveraged our understanding of the myeloid contribution to a multitude of diseases. Here we use CX3CR1 expression as a means to identify a novel nongranulocytic CX3CR1-negative myeloid population that is functionally distinct from the previously described CX3CR1-positive cellular subsets within the CD11b-positive cellular compartment of ascites from ovarian tumor-bearing mice. We functionally identify CX3CR1-negative cells as myeloid suppressor cells and as a cellular subset with pathological specificity. Importantly, the CX3CR1-negative cells exhibit early IL-10 production in the ovarian tumor microenvironment, which we have shown to be critically tied to suppression and additional myeloid-derived suppressor cell accumulation, and we now show that this cellular population actively contributes to tumor progression. Furthermore, we demonstrate that the CX3CR1-negative population is derived fr...Continue Reading

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Citations

May 27, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·David Kung-Chun ChiuCarmen Chak-Lui Wong
Jan 5, 2017·Viral Immunology·Megan A O'ConnorWilliam R Green
Dec 7, 2016·Oncology Reports·Caterina MusolinoSebastiano Gangemi
Feb 23, 2019·Frontiers in Immunology·Yungang WangShengjun Wang
Nov 15, 2017·Molecular Cancer Therapeutics·Courtney A PennRonald J Buckanovich
Jul 18, 2020·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Nuray ErinThomas Efferth

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Methods Mentioned

BETA
transgenic
lavages
FACS
ELISA
flow cytometry

Software Mentioned

FlowJo

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