PMID: 25744829Mar 7, 2015Paper

CXCL12α and CXCL12β stimulate the migration of cultured LX-2 hepatic stellate cells

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Xingxing YuanJinli Lou

Abstract

To investigate the influence of chemokine (C-X-C motif) ligand 12α (CXCL12α) and CXCL12β on migration of hepatic stellate cells (HSCs). The cultured human LX-2 HSCs in vitro were divided into control group, platelet-derived growth factor-BB (PDGF-BB) group (10 ng/mL), CXCL12α group (50, 100, 200 ng/mL) and CXCL12β group (50, 100, 200 ng/mL). Transwell™ assay was used to observe the effect of CXCL12α and CXCL12β on migration of LX-2 cells. Expression of chemokine (C-X-C motif) receptor 4 (CXCR4) in LX-2 cells after treated with or without recombinant human CXCL12 was examined by Western blotting. The number of migration cells in each group were as follows, control group (66.33 ± 11.43), PDGF-BB group (127.47 ± 31.68), 50, 100, 200 ng/mL CXCL12α group (106.13 ± 12.94, 125.87 ± 17.00, 137.07 ± 21.03), 50, 100, 200 ng/mL CXCL12β group (103.80 ± 11.26, 122.33 ± 19.46, 124.40 ± 15.16). Both CXCL12α and CXCL12β induced the migration of LX-2 cells, and migratory response increased in a dose-dependent manner. Western blotting demonstrated that CXCR4 was expressed in LX-2 cells and expression level of CXCR4 protein was not altered after stimulation with exogenous CXCL12. Both CXCL12α and CXCL12β could induce the migration of HSCs.

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