cxcl12-engineered endothelial progenitor cells enhance neurogenesis and angiogenesis after ischemic brain injury in mice

Stem Cell Research & Therapy
Yaning LiYongting Wang

Abstract

Ischemic stroke causes a multitude of brain damage. Neurovascular injury and myelin sheath degradation are two manifestations of ischemic brain damage. Therapeutic strategies aiming only at repairing the neural components or the vessels cannot efficiently restore neurological function. Endothelial progenitor cells (EPCs) have the advantages of both promoting angiogenesis and secreting trophic factors that would promote neurogenesis. Chemokine cxcl12 gene therapy has also been shown to promote angiogenesis, neurogenesis, and remyelination, attracting EPCs, neural progenitor cells, and oligodendrocyte progenitor cells (OPCs) to the injured sites of the brain. In this work, we tested whether these two therapeutics can be combined by genetically engineering the EPCs with cxcl12 to harness the synergistic effects of these two interventions. We used lentivirus (LV) to deliver cxcl12 gene into human umbilical cord blood EPCs to generate the engineered CXCL12-EPCs, which were then delivered into the perifocal region at 1 week after permanent middle cerebral artery occlusion to investigate the effects of CXCL12-EPCs on the functional recovery and angiogenesis, neurogenesis, and remyelination in ischemic stroke mice. Green fluorescent pr...Continue Reading

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Citations

Dec 5, 2019·International Journal of Molecular Sciences·Roxana SurugiuAurel Popa-Wagner
Nov 5, 2019·Current Stem Cell Research & Therapy·L Vinod Kumar ReddyDwaipayan Sen
Dec 24, 2019·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yuanyuan MaGuo-Yuan Yang
Aug 23, 2018·American Journal of Physiology. Cell Physiology·Gema EsquivaAnna Rosell
Feb 18, 2020·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Lu JiangXiaoming Hu
Jan 10, 2021·Stem Cell Reviews and Reports·Lacy E LowryJames A Bynum
Apr 2, 2020·Current Stem Cell Research & Therapy·Xiaojie BaiHong Zhang
Sep 30, 2018·Advanced Drug Delivery Reviews·Austin P VeithAaron B Baker
Jul 4, 2021·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Ji LiDa-Wei Li
Aug 28, 2021·Tissue Engineering and Regenerative Medicine·Ji-Hua LiMin Yao
Jan 4, 2022·Journal of Biochemical and Molecular Toxicology·Huiyi HuoZhiguang Mai

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Methods Mentioned

BETA
flow
transfection
PCR
flow cytometry
FACS

Software Mentioned

Image Pro Plus
GraphPad
ImageJ
GraphPad Prism

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