CXCL13 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Inhibiting miR-23a Expression

Stem Cells International
Feng TianFei Wang

Abstract

CXC chemokines are essential for osteogenic differentiation of bone mesenchymal stem cells (BMSCs) for use in bone tissue engineering and regenerative medicine in clinical settings. However, an accurate understanding of the underlying mechanisms is still needed. In this study, we analyzed the effects of CXC chemokine ligand-13 (CXCL13) on osteogenic differentiation of rat BMSCs and initiated a preliminary discussion on possible mechanisms. BMSCs were isolated from bone marrow of rat and incubated with CXCL13 recombinant protein in differentiation medium. The main osteogenesis indexes were alkaline phosphatase (ALP) activity and calcium nodes. Expression of Runx2 and CXCR5 was determined using western blot, while miRNAs were determined with quantitative-RT-PCR. Si-CXCR5 was transfected into MSCs to silence CXCR5. A miRNA-23a mimic was transfected into BMSCs for overexpression of miRNA-23a. Recombinant CXCL13 induced ALP activity, deposition of calcium salts, and formation of calcium nodes, and it also increased expression of Runx2. The expression of recombinant CXCL13 suppressed expression of miRNA-23a. Overexpression of miR-23a reversed CXCL13 induced-osteogenic differentiation of BMSCs and expression of Runx2. Recombinant CXCL...Continue Reading

References

Sep 16, 2000·Experimental Hematology·R J Deans, A B Moseley
Jan 24, 2009·Nature Reviews. Molecular Cell Biology·Vamsi K Gangaraju, Haifan Lin
Aug 4, 2009·Cell and Tissue Research·Toshihisa Komori
Jun 17, 2010·Journal of Orthopaedic Surgery and Research·Chi Zhang
Oct 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mohammad Q HassanJane B Lian
Nov 10, 2010·Expert Opinion on Biological Therapy·Timothy J MyersAnna Spagnoli
Jan 5, 2011·Sarcoma·J W MartinJ A Squire
Jun 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ying ZhangGary S Stein
Sep 6, 2012·Journal of Cellular Physiology·Claudia M J LuceroMario A Galindo
Apr 3, 2014·Archives of Toxicology·Walid ZaherMoustapha Kassem

❮ Previous
Next ❯

Citations

Oct 25, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhen-Wei LiXu Zhu
Oct 4, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Boran CaoWenbo Wang
Apr 3, 2019·Bone Research·Andreia Machado SilvaMaria Inês Almeida
Jun 30, 2019·Stem Cell Research & Therapy·Jicheng WangZhi Yi
Feb 21, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiu-Mei ZhuangKai-Fang Yuan
May 3, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Cheng JuZhiping Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
Assay
PCR
transfection
transfecting

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.