CXCL16 Induces the Progression of Pulmonary Fibrosis through Promoting the Phosphorylation of STAT3

Canadian Respiratory Journal : Journal of the Canadian Thoracic Society
Sheng ZuoShaojun Yin

Abstract

The transmembrane chemokine (C-X-C motif) ligand 16 (CXCL16) plays a vital role in the pathogenesis of organ fibrosis, including the liver and kidney. However, the detailed biological function of CXCL16 is still not fully understood in the progression of pulmonary fibrosis (PF). The aim of present study is to examine the function of CXCL16 in PF. In this study, we constructed the PF model on mouse by using bleomycin and analyzed the effect of the mouse recombinant protein CXCL16 on mouse lung fibroblast L929 (LF) as well. To further examine the connection between CXCL16 and STAT3 in mouse LF cells, the STAT3 inhibitor AG490 was utilized to inhibit the expression of STAT3. Meanwhile, lipopolysaccharide was used to enhance the phosphorylation of STAT3 (p-STAT3) in mouse LF cells. Our results indicated that the level of CXCL16/CXCR6 was significantly upregulated in the mouse PF model. Moreover, the level of p-STAT3 was also promoted. In addition, the mouse recombinant protein CXCL16 not only contributed to the proliferation of mouse LF cells but also induced the expression of p-STAT3 in LF cells. However, the effect of CXCL16 was deeply abolished by the STAT3 inhibitor AG490 in LF cells. Meanwhile, the antibody of CXCL16 deeply re...Continue Reading

References

Jun 19, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·M P KeaneR M Strieter
Nov 1, 2002·Current Opinion in Rheumatology·Hironobu Ihn
Jun 6, 2003·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Amy W. Rachfal, David R. Brigstock
Aug 6, 2011·Journal of the American Society of Nephrology : JASN·Gang ChenYanlin Wang
Feb 15, 2012·Cellular Signalling·Johannes G BodeDieter Häussinger
Jul 10, 2012·Nature Medicine·Thomas A Wynn, Thirumalai R Ramalingam
Aug 2, 2012·The Journal of Clinical Investigation·Paul W NobleDianhua Jiang
Aug 21, 2013·Annals of the American Thoracic Society·Karen C Patterson, Mary E Strek
Dec 12, 2013·American Journal of Respiratory Cell and Molecular Biology·Elizabeth F RedenteDavid W H Riches
Aug 31, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thanh-Thuy T LeMichael R Blackburn
Sep 2, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mesias PedrozaSandeep K Agarwal
Nov 13, 2015·Endocrine-related Cancer·Sun Wook ChoYoung Joo Park
Dec 2, 2015·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Chang-hong LiXiang-yuan Liu
Feb 27, 2016·Current Protein & Peptide Science·Amit Kumar SrivastavaRajnish Srivastava
Mar 19, 2016·Applied Immunohistochemistry & Molecular Morphology : AIMM·Shaimaa ElzamlyZakaria Y Abd Elmageed
May 19, 2016·Oncotarget·Hua LiangYanlin Wang
Apr 7, 2017·Science Translational Medicine·Pauline DésogèrePeter Caravan
May 10, 2017·Biochemical and Biophysical Research Communications·Yanna YeFanghua Gong
Jan 22, 2018·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Kun LiangKe Hu
Feb 8, 2018·Respiratory Research·Javier MilaraJulio Cortijo
Aug 21, 2018·Inflammation and Regeneration·Naohiro TodaHideki Yokoi

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
PCR
protein assay

Software Mentioned

GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.