CXCL7-Mediated Stimulation of Lymphangiogenic Factors VEGF-C, VEGF-D in Human Breast Cancer Cells.

Journal of Oncology
Minghuan YuMary Ann Kosir

Abstract

Increased expression of lymphangiogenesis factors VEGF-C/D and heparanase has been correlated with the invasion of cancer. Furthermore, chemokines may modify matrix to facilitate metastasis, and they are associated with VEGF-C and heparanase. The chemokine CXCL7 binds heparin and the G-protein-linked receptor CXCR2. We investigated the effect of CXCR2 blockade on the expression of VEGF-C/D, heparanase, and on invasion. CXCL7 siRNA and a specific antagonist of CXCR2 (SB225002) were used to treat CXCL7 stably transfected MCF10AT cells. Matrigel invasion assays were performed. VEGF-C/D expression and secretion were determined by real-time PCR and ELISA assay, and heparanase activity was quantified by ELISA. SB225002 blocked VEGF-C/D expression and secretion (P < .01). CXCL7 siRNA knockdown decreased heparanase (P < .01). Both SB225002 and CXCL7 siRNA reduced the Matrigel invasion (P < .01). The MAP kinase signaling pathway was not involved. The CXCL7/CXCR2 axis is important for cell invasion and the expression of VEGF-C/D and heparanase, all linked to invasion.

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Citations

Aug 29, 2014·Journal of Immunology Research·M Isabel Palacios-ArreolaJorge Morales-Montor
May 12, 2015·BBA Clinical·Merel M KoopmansDiederik van de Beek
Nov 26, 2013·Best Practice & Research. Clinical Anaesthesiology·Caroline P LeErica K Sloan
Aug 30, 2017·British Journal of Cancer·Maeva DufiesGilles Pagès
Jan 23, 2018·Cancer Medicine·Qiang DuQi Wang
Dec 29, 2018·International Journal of Molecular Sciences·Elisabetta MarcuzziBianca Calì
Dec 18, 2013·Cancer Research·Renaud GrépinGilles Pagès
Mar 26, 2016·Cancer Research·Bassem D KhalilJonathan M Backer
Feb 13, 2021·International Journal of Molecular Sciences·Salim GhannoumAlvaro Köhn-Luque

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Methods Mentioned

BETA
transfection
flow cytometry
enzyme-linked immunosorbent assay
Assay
protein assay
ELISA

Software Mentioned

GraphPad
ECL
GraphPad Prism

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