PMID: 9420285Jan 7, 1998Paper

CXCR-4 is expressed by primary macrophages and supports CCR5-independent infection by dual-tropic but not T-tropic isolates of human immunodeficiency virus type 1

Journal of Virology
Y YiR G Collman

Abstract

Primary macrophages are infected by macrophage (M)-tropic but not T-cell line (T)-tropic human immunodeficiency virus type 1 (HIV-1) strains, and CCR5 and CXCR-4 are the principal cofactors utilized for CD4-mediated entry by M-tropic and T-tropic isolates, respectively. Macrophages from individuals homozygous for an inactivating mutation of CCR5 are resistant to prototype M-tropic strains that depend on CCR5 but are permissive for a dual-tropic isolate, 89.6, that can use both CCR5 and CXCR-4, as well as CCR2b, CCR3, and CCR8. Here we show that 89.6 entry into CCR5-deficient macrophages is blocked by an anti-CXCR-4 antibody and by the CXCR-4-specific chemokine SDF but not by the ligands to CCR2b or CCR3. Reverse transcription-PCR demonstrated expression of CXCR-4 but not CCR3 or CCR8 in macrophages, while CCR2b was variable. Macrophage surface expression of CXCR-4 was confirmed by immunofluorescence staining and flow cytometry. Thus, CXCR-4 is expressed by primary macrophages and functions as a cofactor for entry by dual-tropic but not T-tropic HIV-1 isolates, and macrophage resistance to T-tropic strains does not result from a lack of the T-tropic entry cofactor CXCR-4. Since CXCR-4 on macrophages can be used by some but not o...Continue Reading

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Citations

Mar 19, 1999·AIDS Research and Human Retroviruses·N SeddikiA G Hovanessian
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·B LeeR W Doms
Mar 1, 2000·Multiple Sclerosis : Clinical and Laboratory Research·G X ZhangA M Rostami
May 16, 2001·Arthritis and Rheumatism·G H YuanK Nishioka
Feb 13, 2002·AIDS Research and Human Retroviruses·Krystyna Konopka, Nejat Düzgüneş
Jun 11, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alessia VeraniDonata Vercelli
Jan 31, 2002·AIDS·A Brelot, M Alizon
Dec 29, 2005·AIDS Research and Human Retroviruses·Luke Elizabeth HannaSoumya Swaminathan
Mar 27, 2007·Circulation Journal : Official Journal of the Japanese Circulation Society·Yu MisaoHisayoshi Fujiwara
May 20, 1999·Molecular Membrane Biology·T L Hoffman, R W Doms
Aug 13, 1999·AIDS Research and Human Retroviruses·S HibbittsP R Clapham
Feb 13, 2002·AIDS Research and Human Retroviruses·Youssef BakriAbdelaziz Benjouad
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·H XiaoK T Jeang
Apr 1, 1999·The Journal of Infectious Diseases·R G Collman, Y Yi
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Jun 29, 2004·Journal of Virology·Cristina PastoreDonald E Mosier
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