CXCR4 and CXCR7 Mediate TFF3-Induced Cell Migration Independently From the ERK1/2 Signaling Pathway

Investigative Ophthalmology & Visual Science
Julia DieckowFriedrich P Paulsen

Abstract

Trefoil factor family (TFF) peptides, and in particular TFF3, are characteristic secretory products of mucous epithelia that promote antiapoptosis, epithelial migration, restitution, and wound healing. For a long time, a receptor for TFF3 had not yet been identified. However, the chemokine receptor CXCR4 has been described as a low affinity receptor for TFF2. Additionally, CXCR7, which is able to heterodimerize with CXCR4, has also been discussed as a potential TFF2 receptor. Since there are distinct structural similarities between the three known TFF peptides, this study evaluated whether CXCR4 and CXCR7 may also act as putative TFF3 receptors. We evaluated the expression of both CXCR4 and CXCR7 in samples of human ocular surface tissues and cell lines, using RT-PCR, immunohistochemistry, and Western blot analysis. Furthermore, we studied possible binding interactions between TFF3 and the receptor proteins in an x-ray structure-based modeling system. Functional studies of TFF3-CXCR4/CXCR7 interaction were accomplished by cell culture-based migration assays, flow cytometry, and evaluation of activation of the mitogen-activated protein (MAP) kinase signaling cascade. We detected both receptors at mRNA and protein level in all an...Continue Reading

Citations

Mar 10, 2017·Cell Death & Disease·Carlos HanischSoroush Sharbati
Oct 30, 2019·International Journal of Molecular Sciences·Till HoubenWerner Hoffmann
May 24, 2017·World Journal of Gastroenterology : WJG·Qi XinWei Liu
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Sep 5, 2019·International Journal of Molecular Sciences·Katharina KrügerKolja Gelse
Jan 29, 2021·World Journal of Gastroenterology : WJG·Yun HuangShi-Nan Nie
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