PMID: 9658135Jul 11, 1998Paper

CXCR4 is required by a nonprimate lentivirus: heterologous expression of feline immunodeficiency virus in human, rodent, and feline cells

Journal of Virology
Eric M Poeschla, David J Looney

Abstract

A heterologous feline immunodeficiency virus (FIV) expression system permitted high-level expression of FIV proteins and efficient production of infectious FIV in human cells. These results identify the FIV U3 element as the sole restriction to the productive phase of replication in nonfeline cells. Heterologous FIV expression in a variety of human cell lines resulted in profuse syncytial lysis that was FIV env specific, CD4 independent, and restricted to cells that express CXCR4, the coreceptor for T-cell-line-adapted strains of human immunodeficiency virus. Stable expression of human CXCR4 in CXCR4-negative human and rodent cell lines resulted in extensive FIV Env-mediated, CXCR4-dependent cell fusion and infection. In feline cells, stable overexpression of human CXCR4 resulted in increased FIV infectivity and marked syncytium formation during FIV replication or after infection with FIV Env-expressing vectors. The use of CXCR4 is a fundamental feature of lentivirus biology independent of CD4 and a shared cellular link to infection and cytopathicity for distantly related lentiviruses that cause AIDS. Their conserved use implicates chemokine receptors as primordial lentivirus receptors.

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Citations

May 20, 1999·Molecular Membrane Biology·B J Willett, M J Hosie
Jan 29, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D L ThompsonR Kumar
Jan 11, 2003·Clinical and Diagnostic Laboratory Immunology·Mariko KohmotoTakayuki Miyazawa
May 14, 2010·Journal of Virology·Melissa A SternEric M Poeschla
May 3, 2001·Journal of Virology·S C FreyJ I Mullins
Feb 14, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Arnt-Ove Hovden, Maja A Sommerfelt
Aug 17, 2004·Journal of Virology·Sohela de RozièresJohn H Elder
Nov 25, 2005·Journal of Virology·Dyana T SaenzEric M Poeschla
Jul 18, 2009·PLoS Pathogens·Anne M MeehanEric M Poeschla
May 25, 2012·Journal of Virology·Iris KemlerEric Poeschla
May 22, 2002·Journal of Virology·Leslie A Obert, Edward A Hoover
Nov 5, 2002·Journal of Virology·Iris KemlerEric M Poeschla
Sep 5, 2001·Journal of Virology·T WhitwamE Poeschla
Feb 23, 2012·Viruses·Hind J FadelEric M Poeschla
Feb 26, 2000·Annual Review of Genetics·H TangF Wong-Staal
Dec 19, 2001·Human Gene Therapy·N LoewenE M Poeschla
Apr 16, 2010·Journal of Virology·Iris KemlerEric M Poeschla
Jul 10, 1999·Journal of Virology·S W DowE A Hoover

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