Cyanase-mediated utilization of cyanate in Pseudomonas fluorescens NCIB 11764.

Applied and Environmental Microbiology
D A Kunz, O Nagappan

Abstract

Pseudomonas fluorescens NCIB 11764 was capable of utilizing cyanate (OCN-) as a sole nitrogen source for growth. Crude cell extracts from cells grown on cyanate, but not on ammonium sulfate, were induced for an enzyme catalyzing cyanate conversion to ammonia. Enzymatic activity was shown to be bicarbonate dependent and specific for cyanate as a substrate, suggesting that cyanate utilization in this organism is facilitated by an enzyme resembling cyanase (cyanate amidohydrolase; EC 3.5.5.3), as described previously in Escherichia coli and Flavobacterium sp.

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Citations

Feb 5, 2005·Applied and Environmental Microbiology·Víctor M Luque-AlmagroRafael Blasco
May 18, 2019·Environmental Science and Pollution Research International·Xiao-Zhang YuQing Zhang
Aug 19, 2008·Applied and Environmental Microbiology·Víctor M Luque-AlmagroRafael Blasco
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Dec 14, 2018·World Journal of Microbiology & Biotechnology·Tomas Linder
Mar 8, 2017·Applied Microbiology and Biotechnology·Jason M ParkMichael J Benedik
Mar 28, 2017·Environmental Science and Pollution Research International·Rashad Kebeish, Omar Al-Zoubi
Dec 1, 1990·Applied and Environmental Microbiology·J Silva-AvalosD A Kunz
Dec 29, 2017·Environmental Science & Technology·Irina KurashovaAlexey Kamyshny
Sep 18, 2008·Fungal Genetics and Biology : FG & B·Skander Elleuche, Stefanie Pöggeler

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