Abstract
Several cyanide-tolerant microorganisms have been selected from alkaline wastes and soils contaminated with cyanide. Among them, a fungus identified as Fusarium solani IHEM 8026 shows a good potential for cyanide biodegradation under alkaline conditions (pH 9.2 to 10.7). Results of K(sup14)CN biodegradation studies show that fungal metabolism seems to proceed by a two-step hydrolytic mechanism: (i) the first reaction involves the conversion of cyanide to formamide by a cyanide-hydrolyzing enzyme, cyanide hydratase (EC 4.2.1.66); and (ii) the second reaction consists of the conversion of formamide to formate, which is associated with fungal growth. No growth occurred during the first step of cyanide degradation, suggesting that cyanide is toxic to some degree even in cyanide-degrading microorganisms, such as F. solani. The presence of organic nutrients in the medium has a major influence on the occurrence of the second step. Addition of small amounts of yeast extract led to fungal growth, whereas no growth was observed in media containing cyanide as the sole source of carbon and nitrogen. The simple hydrolytic detoxification pathway identified in the present study could be used for the treatment of many industrial alkaline efflu...Continue Reading
References
Jan 1, 1992·Biodegradation·S A Raybuck
Nov 1, 1992·Archives of Biochemistry and Biophysics·P WangH D VanEtten
Jun 1, 1991·Applied and Environmental Microbiology·K IngvorsenS E Godtfredsen
Jan 1, 1988·Ciba Foundation Symposium·K IngvorsenR T Tsuchiya
Sep 1, 1986·Annals of Emergency Medicine·A H Hall, B H Rumack
Jan 1, 1986·Advances in Microbial Physiology·C J Knowles, A W Bunch
Aug 1, 1972·Archives of Biochemistry and Biophysics·W E Fry, R L Millar
Jul 1, 1994·Plant Physiology·L McIntosh
Jul 1, 1994·Microbiology·D A KunzJ L Chen
Aug 1, 1993·Journal of General Microbiology·M J ClunessC O'Reilly
Mar 1, 1996·Applied Microbiology and Biotechnology·G R BabuK D Chapatwala
Jan 5, 1989·Biotechnology and Bioengineering·D J Richards, W K Shieh
Mar 15, 1992·Biotechnology and Bioengineering·S BasheerJ R Bourne
Citations
Jan 19, 2006·Biochemical Society Transactions·M-J HuertasM-D Roldán
May 10, 2006·Antonie van Leeuwenhoek·Joanne Baxter, Stephen P Cummings
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Luigia SabatiniFrancesca Bruscolini
Jan 1, 2012·Insects·Scott M GeibKelli Hoover
Sep 28, 2002·FEMS Microbiology Letters·Hyouk K KwonJong M Park
Nov 14, 2006·Eye & Contact Lens·Ruth A RosenthalRalph P Stone
Aug 11, 2011·International Journal of Systematic and Evolutionary Microbiology·Thabiso E MotaungCarolina H Pohl
Feb 2, 2000·Applied and Environmental Microbiology·K YoshikawaH Sano
Feb 11, 2005·Applied Microbiology and Biotechnology·Dakshina M JandhyalaMichael J Benedik
Feb 5, 2005·Applied and Environmental Microbiology·Víctor M Luque-AlmagroRafael Blasco
Dec 29, 2006·Small·Atul BhardeMurali Sastry
Jul 22, 2005·Letters in Applied Microbiology·C M KaoS C Chen
Feb 5, 2014·The Journal of General and Applied Microbiology·Maegala Nallapan ManiyamAnthony E G Cass
Jun 13, 2014·Journal of Environmental Health Science & Engineering·Shabnam MirizadehZahra Ghobadi Nejad
May 22, 2019·Current Microbiology·Ramasamy MahendranGayathri Muthusamy
Sep 29, 2019·Plant Physiology and Biochemistry : PPB·Amira Susana NievaOscar Adolfo Ruiz
Jun 22, 2019·Plant, Cell & Environment·Almuth HammerbacherJonathan Gershenzon
Dec 30, 2017·Applied Microbiology and Biotechnology·Thandeeswaran MurugesanAngayarkanni Jayaraman
Oct 20, 2005·Journal of Bioscience and Bioengineering·M D Adjei, Y Ohta
Jul 29, 2008·Journal of Hazardous Materials·Rajesh Roshan DashChandrajit Balomajumder