Cyanide stimulation of tri-N-butyltin mediated hemolysis

Journal of Applied Toxicology : JAT
B H GrayL H Lee

Abstract

The effects of tributyltin and sodium cyanide on hemolysis in human erythrocytes are described. Tributyltin has a sharp cut-off concentration for induction of hemolysis. A 5 microM concentration of tributyltin induces hemolysis and 1 microM or less does not in erythrocyte suspensions with 2.3 X 10(8) cells per ml. The kinetics for tributyltin-induced hemolysis are sigmoidal indicating a complex molecular mechanism leading to lysis. Ten mM sodium cyanide plus 1 microM tributyltin does not stimulate hemolysis rates above levels observed with 10 mM sodium cyanide alone. Five mM sodium cyanide plus hemolytic concentrations of tributyltin stimulates hemolysis rates synergistically compared with either cyanide or tributyltin alone. Ultrastructurally, hemolytic concentrations of tributyltin can be visualized in the electron microscope by osmium staining during fixation as electron-dense spheres penetrating the lipid bilayer of the erythrocyte plasma membrane. Ten mM sodium cyanide plus 25 microM tributyltin increases slightly the size of osmiophilic structures in erythrocyte membranes compared with those spheres seen in cells exposed to 25 microM tributyltin alone. Sodium cyanide is the only compound tested that stimulates tributyltin...Continue Reading

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Citations

Dec 1, 1987·Toxicology·B H GrayL H Lee
Oct 1, 1988·Toxicology Letters·R M ZuckerE J Massaro
Feb 14, 2006·Biochimica Et Biophysica Acta·Antonio OrtizFrancisco J Aranda
Oct 1, 1986·Journal of Applied Toxicology : JAT·B H GrayC Flemming

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