Cyanidin-3-O-glucoside restores insulin signaling and reduces inflammation in hypertrophic adipocytes

Archives of Biochemistry and Biophysics
Maria Sofia MoloniaM Cristani

Abstract

Obesity is a metabolic disorder characterized by excess adipose tissue, macrophages infiltration, and inflammation which in turn lead to insulin-resistance. Epidemiological evidences reported that anthocyanins possess not only high antioxidant and antiinflammatory activities, but also improve metabolic complications associated with obesity. The aim of this work was to evaluate the in vitro beneficial effects of cyanidin-3-O-glucoside (C3G) in counteracting inflammation and insulin-resistance in 3T3-L1 hypertrophic adipocytes exposed to palmitic acid (PA). In the present study murine 3T3-L1 adipocytes were pretreated with C3G for 24 h and then exposed to palmitic acid (PA) for 24 h. Real-time PCR, western blotting analysis and Oil Red O staining were applied for investigating the mechanism involved in adipocytes dysfunction. C3G pretreatment reduced lipid accumulation, PPARγ pathway and NF-κB pathway induced by PA in murine adipocytes. In addition, our data demonstrated that PA reduced insulin signaling via IRS-1 Ser307phosphorylation while C3G dose-dependently improved insulin sensitivity restoring IRS-1/PI3K/Akt pathway. Furthermore, C3G improved adiponectin mRNA levels altered by PA in 3T3-L1 murine and SGBS human adipocytes....Continue Reading

Citations

Jun 3, 2021·Biomolecules·Ioana MozosAtanas G Atanasov
Jul 3, 2021·International Journal of Molecular Sciences·Shiyao ZhangSiyu Chen
Jul 3, 2021·Cells·María Ángeles Martín, Sonia Ramos
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jin-Nam KimHye-Kyeong Kim
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aleksandra Kozłowska, Tomasz Dzierżanowski

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