Cyanobacteria carrying an smt-lux transcriptional fusion as biosensors for the detection of heavy metal cations

Journal of Industrial Microbiology
J L ErbeL M Hall

Abstract

The metal-responsive smt operator/promoter region of Synechococcus PCC7942 was fused to the luxCDABE genes of Vibrio fischeri. Plasmid DNA (pJLE23) carrying this fusion conferred metal ion-inducible luminescence to transformed cyanobacteria. Synechococcus PCC7942 (pJLE23) was sensitive to ZnCl2 concentrations within a range of 0.5-4 microM as demonstrated by induction of luminescence. Trace levels of CuSO24 and CdCl2 were also detected.

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Citations

Jun 1, 2011·Analytical and Bioanalytical Chemistry·Roberta BraynerClémence Sicard
Jun 6, 2003·Trends in Biotechnology·Till Bachmann
Apr 20, 2010·Chemical Communications : Chem Comm·Christophe F MeunierBao-Lian Su
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Mar 19, 2020·Chembiochem : a European Journal of Chemical Biology·Jittima PhonbupphaPimchai Chaiyen
Feb 28, 2021·Environmental Science and Pollution Research International·Sindhunath Chakraborty, Arun Kumar Mishra
Apr 13, 2021·World Journal of Microbiology & Biotechnology·Syed Azmal AliGurjeet Kaur
Apr 4, 2003·Analytical Biochemistry·Israel BiranDavid R Walt
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