PMID: 3748845Jan 1, 1986Paper

Cycle of VIP in the human transformed colonic epithelial cells (HT-29) in culture

Peptides
J C MarieG Rosselin

Abstract

The kinetics of VIP processing in different compartments (medium, plasma membrane and intracellular) by HT-29 cells was studied using direct biochemical methods and a homogeneous monoiodinated VIP. The compartmental radioactivity was characterized by HPLC fractionation and specific receptor binding. VIP once bound to the cell surface remains intact and is rapidly and maximally internalized (less than 10 min) at 37 degrees C. Then two different processes occur: (1) release of degradation products 125I and monoiodinated tyrosine in the medium; (2) VIP remains intact in the cells representing 67.2 +/- 4.7% of total radioactivity up to 90 min. The overall processing of VIP is time- and temperature-dependent and maximal internalization of VIP with minimal medium release is observed at 20 degrees C. Our results demonstrate a receptor mediated internalization of VIP and that at least two intracellular pathways may exist in the cycle of VIP. One is associated with a complete degradation of VIP detected in the extracellular medium and is optimal at 37 degrees C. The other results in the presence of intact intracellular VIP and is optimal at 20 degrees C.

References

Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M LaburtheG Rosselin
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B A Yankner, E M Shooter
Jan 1, 1985·The American Journal of Physiology·D A Wall, T Maack
Jul 7, 1973·Lancet·S R BloomA G Pearse
Apr 1, 1981·The Journal of Clinical Endocrinology and Metabolism·J P BroyartG Rosselin
Jan 1, 1981·Annual Review of Physiology·I H Pastan, M C Willingham
Aug 1, 1982·Molecular and Cellular Endocrinology·G RosselinW Rostène
Sep 1, 1982·Journal of Cellular Physiology·W K Miskimins, N Shimizu
Dec 15, 1981·Biochemical and Biophysical Research Communications·J N LaverriereA Tixier-Vidal
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

❮ Previous
Next ❯

Citations

Dec 1, 1989·European Journal of Clinical Investigation·W BernhardP Von Wichert
Jan 1, 1988·Annals of the New York Academy of Sciences·G RosselinJ C Marie

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.