Cycleave polymerase chain reaction method is practically applicable for V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS)/V-raf murine sarcoma viral oncogene homolog B1 (BRAF) genotyping in colorectal cancer

Translational Research : the Journal of Laboratory and Clinical Medicine
Tomoya YokotaY Yatabe

Abstract

Activating V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) and V-raf murine sarcoma viral oncogene homolog B1 (BRAF) gene mutations are important predictive markers for antiepidermal growth factor receptor chemotherapy in colorectal cancer (CRC). However, a rapid and accurate assay for KRAS/BRAF mutation detection from routine pathological specimens is lacking in clinical practice. We applied the cycleave polymerase chain reaction (PCR) method to routine KRAS/BRAF genotyping of CRC patients at our institution from 2001 to 2009. The accuracy of cycleave PCR genotyping was shown by the high concordance with reverse transcriptase-PCR-coupled direct sequencing. KRAS gene mutations were analyzed successfully from small biopsy or cytology specimens. Although some surgical specimens could not be evaluated by cycleave PCR, corresponding biopsy specimens could be used instead. This PCR failure observed for some biopsy specimens may have been a result of the use of formalin fixation, as overfixation of surgical specimens by formalin impaired PCR amplification. In conclusion, cycleave PCR is practically applicable to KRAS/BRAF genotyping using small amounts of biopsied tumor cells. Care must be taken in the selection of pathol...Continue Reading

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Citations

Mar 26, 2011·International Journal of Clinical Oncology·Ayako MizotaKei Muro
Jun 10, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Kohei ShitaraKei Muro
Nov 9, 2017·Pathology Oncology Research : POR·Karol PolomFranco Roviello
Apr 4, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Jingzhe ZhangWenjun Wang
Feb 23, 2019·Histopathology·Chiaki KondohYasushi Yatabe
May 26, 2016·Asian Pacific Journal of Cancer Prevention : APJCP·Mehrdad PayandehMaryam Shahbazi
Jan 28, 2015·World Journal of Gastroenterology : WJG·Krittiya KorphaisarnCharuwan Akewanlop
Nov 23, 2017·Cardiovascular and Interventional Radiology·Takaaki HasegawaYasushi Yatabe
Dec 29, 2020·Clinical Case Reports·Hidenori SuzukiNobuhiro Hanai

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