Cyclic-β-glucans of Rhizobium (Sinorhizobium) sp. strain NGR234 are required for hypo-osmotic adaptation, motility, and efficient symbiosis with host plants

FEMS Microbiology Letters
Jérémie Gay-FraretA Le Quéré

Abstract

Cyclic-β-glucans (CβG) consist of cyclic homo-polymers of glucose that are present in the periplasmic space of many Gram-negative bacteria. A number of studies have demonstrated their importance for bacterial infection of plant and animal cells. In this study, a mutant of Rhizobium (Sinorhizobium) sp. strain NGR234 (NGR234) was generated in the cyclic glucan synthase (ndvB)-encoding gene. The great majority of CβG produced by wild-type NGR234 are negatively charged and substituted. The ndvB mutation abolished CβG biosynthesis. We found that, in NGR234, a functional ndvB gene is essential for hypo-osmotic adaptation and swimming, attachment to the roots, and efficient infection of Vigna unguiculata and Leucaena leucocephala.

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Citations

Mar 22, 2014·PloS One·Masahiro NakajimaMotomitsu Kitaoka
Jun 12, 2013·Biopolymers·Ritika DwivediChristine M Szymanski
May 24, 2016·International Journal of Molecular Sciences·Francisco J López-BaenaJosé M Vinardell
Dec 19, 2014·PloS One·Dulce N Rodríguez-NavarroJosé M Vinardell
Jun 14, 2019·Bioscience, Biotechnology, and Biochemistry·Kaito KobayashiHayao Taguchi
Mar 4, 2017·Archives of Microbiology·Niurka MenesesSergio Encarnación
Sep 16, 2020·Journal of Experimental Botany·Alan WankeAlga Zuccaro

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