Cyclic adenosine 3',5'-monophosphate regulates GLUT4 and GLUT1 glucose transporter expression and stimulates transcriptional activity of the GLUT1 promoter in muscle cells

Endocrinology
Francesc ViñalsAntonio Zorzano

Abstract

We have previously reported that innervation-dependent basal contractile activity regulates in an inverse manner the expression of GLUT1 and GLUT4 glucose transporters in skeletal muscle. Based on the facts that muscle innervation decreases and muscle denervation increases cAMP levels, we investigated whether cAMP might mediate the effects of innervation/denervation on glucose transporter expression. Treatment of L6E9 myotubes with 8-bromo-cAMP, forskolin, or monobutyryl-8-bromo-cAMP led to a marked decrease in GLUT4 protein levels; 8-bromo-cAMP also diminished GLUT4 messenger RNA (mRNA), suggesting pretranslational repression. In contrast, L6E9 myoblasts and myotubes responded to 8-bromo-cAMP or forskolin by increasing the cell content of GLUT1 protein. Induction of GLUT1 protein was a consequence of the activation of different mechanisms in myoblast and myotube cells; whereas 8-bromo-cAMP treatment caused a substantial increase in GLUT1 mRNA in myoblasts, no change in GLUT1 mRNA was detected in myotubes. The increase in GLUT1 mRNA in L6E9 myoblasts induced by 8-bromo-cAMP was the result of transcriptional activation, as concluded from transfection analysis of 2.1 kilobases of the rat GLUT1 gene promoter fused to the bacterial...Continue Reading

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Citations

Sep 2, 2009·Muscle & Nerve·Ana Barbara Teixeira Alves-WagnerUbiratan Fabres Machado
Mar 6, 2016·Journal of the Neurological Sciences·Seung Hak LeeShi-Uk Lee
Jan 18, 2006·British Journal of Pharmacology·Julia NevzorovaRoger J Summers
Jan 29, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Luc Leybaert
Oct 12, 2000·Journal of Cellular Physiology·C J Clancey, J E Lever
Jun 7, 2006·Archives of Physiology and Biochemistry·Sonia Fernández-VeledoMargarita Lorenzo
Apr 10, 2015·American Journal of Physiology. Cell Physiology·Jonai Pujol-GiménezMaria Pilar Lostao
Feb 16, 2018·Current Diabetes Reviews·Eric Francelino AndradeLuciano José Pereira
Jul 23, 2002·Journal of Applied Physiology·G Lynis Dohm
Oct 15, 2013·Journal of Pineal Research·Ivonne Bazwinsky-WutschkeElmar Peschke
Dec 18, 2002·The Journal of Biological Chemistry·Cinzia Dello RussoDouglas L Feinstein
Aug 2, 2003·The Journal of Biological Chemistry·Horacio MorenoAntonio Zorzano
Feb 12, 1998·Biochemical and Biophysical Research Communications·O Ezaki

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