Cyclic AMP deficiency negatively affects cell growth and enhances stress-related responses in tobacco Bright Yellow-2 cells

Plant Molecular Biology
Wilma SabettaMaria Concetta De Pinto

Abstract

Cyclic adenosine 3',5'-monophosphate (cAMP) is a recognized second messenger; however, knowledge of cAMP involvement in plant physiological processes originates primarily from pharmacological studies. To obtain direct evidence for cAMP function in plants, tobacco Bright Yellow-2 (BY-2) cells were transformed with the cAMP sponge, which is a genetically encoded tool that reduces cAMP availability. BY-2 cells expressing the cAMP sponge (cAS cells), showed low levels of free cAMP and exhibited growth inhibition that was not proportional to the cAMP sponge transcript level. Growth inhibition in cAS cells was closely related to the precocious inhibition of mitosis due to a delay in cell cycle progression. The cAMP deficiency also enhanced antioxidant systems. Remarkable changes occurred in the cAS proteomic profile compared with that of wild-type (WT) cells. Proteins involved in translation, cytoskeletal organization, and cell proliferation were down-regulated, whereas stress-related proteins were up-regulated in cAS cells. These results support the hypothesis that BY-2 cells sense cAMP deficiency as a stress condition. Finally, many proteasome subunits were differentially expressed in cAS cells compared with WT cells, indicating th...Continue Reading

References

Aug 15, 1991·Biochemical and Biophysical Research Communications·L GangwaniS C Maheshwari
Nov 1, 1991·Trends in Genetics : TIG·A Trewavas, S Gilroy
Aug 21, 1973·Biochemical and Biophysical Research Communications·P RaymondA Pradet
Mar 20, 1974·Biochimica Et Biophysica Acta·B Kessler, R Levinstein
Apr 1, 1993·Archives of Biochemistry and Biophysics·F Kurosaki, A Nishi
Jun 10, 1998·Nature·G L WheelerN Smirnoff
Jul 15, 1998·Plant Physiology·I D VolotovskiM R Knight
Mar 6, 1999·Current Opinion in Plant Biology·R Walden
Nov 26, 1999·FEBS Letters·H EhsanH Van Onckelen
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·A MoutinhoR Malhó
Dec 18, 2001·Plant Physiology·F J Maathuis, D Sanders
Feb 5, 2002·The Plant Cell·Jan SmalleRichard D Vierstra
Feb 14, 2002·Plant Physiology·Qiang LengGerald A Berkowitz
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 5, 2002·Nature Reviews. Molecular Cell Biology·Joseph A Beavo, Laurence L Brunton
Nov 1, 2002·Phytochemistry·Helen RichardsRussell P Newton
Dec 21, 2002·Journal of Experimental Botany·Enrique OlmosEladio Hellín
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Susan L Forsburg
Jun 3, 2004·Electrophoresis·Giovanni CandianoPier Giorgio Righetti
Jun 17, 2004·The Journal of Biological Chemistry·Fouad Lemtiri-Chlieh, Gerald A Berkowitz
Sep 24, 2004·Phytochemistry·Russell P Newton, Christopher J Smith
Mar 28, 2006·Physiology·Viacheslav O Nikolaev, Martin J Lohse
Aug 29, 2007·The Journal of Experimental Medicine·Dale A MouldingAdrian J Thrasher
Sep 18, 2007·Plant Cell Reports·Milena MarsoniCandida Vannini
Sep 20, 2007·The Plant Journal : for Cell and Molecular Biology·John DowdleNicholas Smirnoff
Dec 7, 2007·Plant Physiology·Mansour KarimiPierre Hilson
Jan 30, 2008·Current Protocols in Cell Biology·Federica BrandizziChris Hawes
Nov 18, 2008·Current Opinion in Plant Biology·Toon Cools, Lieven De Veylder
Nov 6, 2009·PloS One·Konstantinos LefkimmiatisAldebaran M Hofer
Nov 26, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wei MaGerald A Berkowitz
Jun 29, 2010·Cell Communication and Signaling : CCS·Chris Gehring
Aug 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Florian BrioudesMohammed Bendahmane
Nov 16, 2010·Plant Cell Reports·L A LomovatskayaL V Dudareva
Jan 6, 2011·Plant Physiology·Christine H Foyer, Graham Noctor

❮ Previous
Next ❯

Citations

Apr 28, 2017·Frontiers in Physiology·Rafael PeláezAlfredo Martínez
Jul 15, 2020·International Journal of Molecular Sciences·Emanuela BlancoMaria Concetta de Pinto
Sep 3, 2020·Plant, Cell & Environment·Annalisa ParadisoMaria Concetta de Pinto
Aug 31, 2021·Frontiers in Plant Science·Inas Al-YounisChris Gehring

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.