Cyclic AMP-dependent modulation of giant depolarizing potentials by metabotropic glutamate receptors in the rat hippocampus

The Journal of Physiology
F StrataE Cherubini

Abstract

1. Intracellular recordings were used to study the role of metabotropic glutamate receptors (mGluRs) in modulating GABA-mediated giant depolarizing potentials (GDPs) in immature rat hippocampal CA3 neurones. 2. The mGluR antagonist (RS)-alpha-methyl-4-carboxyphenylglycine (MCPG, 1 mM) reduced the frequency of GDPs. The broad-spectrum ionotropic glutamate receptor antagonist kynurenic acid (1 mM) blocked GDPs. 3. In the presence of kynurenic acid, both tetanic stimulation of the hilus or bath application of quisqualic acid (1 microM) and trans-1-aminocyclopentane-1,3-dicarboxylic acid (t-ACPD, 20 microM) induced the appearance of GDPs. These effects were antagonized by MCPG (1 mM) or L(+)-2-amino-3-phosphonopropionic acid (L-AP3) and blocked by bicuculline (10 microM). 4. 8-Bromo-cAMP (8-Br-cAMP, 0.3 mM), 3-isobutyl-1-methylxanthine (IBMX, 200 microM) or forskolin (30 microM) mimicked the effects of mGluR agonists on GDPs. The forskolin analogue 1,9-dideoxyforskolin (30 microM), which does not activate adenylate cyclase, was ineffective. 5. Incubation of slices in the presence of the protein kinase A inhibitor Rp-adenosine 3',5'-cyclic monophosphothioate triethylamine (Rp-cAMPS) (500 microM) or superfusion of Rp-cAMPS (20 microM...Continue Reading

Citations

May 18, 2001·Trends in Neurosciences·Y Ben-Ari
Aug 7, 2004·The Journal of Physiology·Rebekah CorlewWilliam J Moody
Sep 30, 1998·The European Journal of Neuroscience·L Menendez de la PridaJ V Sanchez-Andres
Aug 9, 2016·PloS One·Laura A AthertonJack R Mellor
Jul 20, 2007·Journal of Neurophysiology·Adriano Augusto CattaniLaurent Aniksztejn
Oct 12, 2007·Physiological Reviews·Yehezkel Ben-AriRustem Khazipov
May 6, 2017·Frontiers in Neurology·Xin-Xin WangQin-Chi Lu
Feb 11, 1998·Physiological Reviews·F LangD Häussinger

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