PMID: 2511389Jan 1, 1989Paper

Cyclic AMP-dependent protein kinase interferes with GTP gamma S stimulated IP3 formation in differentiated HL-60 cell membranes

Life Sciences
N MisakiY Watanabe

Abstract

The effects of addition of activated cyclic AMP-dependent protein kinase (PKA) on the function of islet-activating protein (IAP)-sensitive GTP-binding (G) protein were studied in the plasma membranes of 3H-inositol-labeled differentiated human leukemic (HL-60) cells. Pretreatment of the membranes with activated PKA (0.1 mg/ml) in the presence of MgATP for 15 min. at 37 degrees C decreased GTP gamma S-stimulated inositol trisphosphate (IP3) formation by about 30%, but had no influence on Ca2+-stimulated IP3 formation. And autoradiography in the phosphorylation experiments of solubilized HL-60 cell membranes by PKA showed some 32P incorporated bands, and among them one of the major bands showed the migration at 40 kDa supporting that the G protein coupling with PI response was phosphorylated by PKA. These results showed that pretreatment with activated PKA inhibited the mediating function of the G protein between the fMLP receptor and phospholipase C by its phosphorylation.

References

Jul 1, 1986·Journal of Molecular and Cellular Cardiology·R W TsienM C Nowycky
Dec 1, 1988·Trends in Neurosciences·D A Eberhard, R W Holz
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A AlbiniM E Lippman
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L M Leeb-LundbergM G Caron

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Citations

Mar 19, 1991·Biochimica Et Biophysica Acta·E MeldrumA Carozzi
Oct 24, 2003·Cell Calcium·Jason I E BruceDavid I Yule
Jul 1, 1993·British Journal of Pharmacology·E MoilanenH Vapaatalo

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