Cyclic AMP formation in rat bone and kidney cells is stimulated equally by parathyroid hormone-related protein (PTHrP) 1-34 and PTH 1-34

Experimental and Clinical Endocrinology
E BlindR Ziegler

Abstract

Parathyroid hormone-related protein (PTHrP) plays a major role in the pathogenesis of humoral hypercalcemia of malignancy. It interacts with the PTH receptor and has therefore a nearly identical effect on bone cells as PTH. However, PTHrP is thought to be less potent than PTH in stimulating adenylate cyclase in canine renal membranes, leading to the hypothesis of a differential efficiency in signal transduction by PTHrP with respect to bone vs kidney. In a homologous model with intact osteoblast-like cells (UMR 106) and primary kidney cells, both from the rat, we have tested N-terminal peptide fragments, based on the rat amino acid sequence 1-34, of PTH and PTHrP. Compared with PTHrP(1-34), rat PTH(1-34) had a similar relative potency in bone cells (85%) and in kidney cells (140%) in its ability to stimulate adenylate cyclase. Human PTH(1-34) was 5.6- to 6.5-fold less potent than rat PTH(1-34) in both cell types. In human osteoblast-like cells (SaOS-2), rat and human PTH were essentially equally potent compared to PTHrP(1-34) (identical sequence in rat and human) in stimulating cAMP accumulation. In conclusion, our study revealed the equipotency of rat PTH(1-34) and PTHrP(1-34) in stimulating intracellular cAMP formation in a h...Continue Reading

Citations

Apr 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Augen A Pioszak, H Eric Xu
Apr 1, 1995·Archives of Physiology and Biochemistry·J Rouffet, J P Barlet
Mar 26, 2015·Frontiers in Endocrinology·Jason S W OnLeo T O Lee
Apr 4, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Archana KamalakarLarry J Suva

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