PMID: 6306141May 1, 1983Paper

Cyclic AMP-induced chloride permeability in the apical membrane of Necturus gallbladder epithelium

The Journal of General Physiology
K U Petersen, L Reuss

Abstract

The effects of theophylline, 8-Br-cAMP, and cAMP on necturus gallbladder epithelium were investigated using microelectrode techniques. Each of these substances depolarized the cell membranes by approximately 15 mV and decreased the apparent ratio of apical to basolateral membrane resistances to a value not significantly different from zero. Examination of the ionic selectivity of the apical membrane by ion substitutions in the mucosal bathing medium revealed a large increase in Cl permeability with no apparent changes in K and Na permeabilities. Intracellular Cl activity ((a)CL(i)) was measured using Cl- sensitive liquid ion-exchanger microelectrodes. Under control conditions, (a)Cl(i) was approximately 20 mM, 2.5 times higher than the value expected for equilibrium distribution ((a)Cl(i/eq). After addition of 8-Br-cAMP, (a)Cl(i) decreased within less than 60 s to approximately 13 mM, a value not significantly different from ((a)Cl(i/eq)). Virtually identical results were obtained with theophylline. Under control conditions, luminal Cl removal caused (a)Cl(i) to fall at an initial rate of 1.8 mM/min, whereas in tissues exposed to 8-Br- cAMP or theophylline a rate of 11.6 mM/min was observed. The apical membrane Cl transference ...Continue Reading

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Citations

May 1, 1990·Pflügers Archiv : European journal of physiology·K U PetersenJ M Winterhager
Aug 1, 1986·Pflügers Archiv : European journal of physiology·F LangM Paulmichl
Jan 1, 1985·Pflügers Archiv : European journal of physiology·K U PetersenJ M Winterhager
Jul 1, 1989·Pflügers Archiv : European journal of physiology·F WehnerK U Petersen
Jan 1, 1986·Pflügers Archiv : European journal of physiology·K Hermansson, K R Spring
Apr 1, 1988·Pflügers Archiv : European journal of physiology·W V BreuerA Rothstein
Apr 1, 1986·Pflügers Archiv : European journal of physiology·M PaulmichlF Lang
Oct 1, 1993·Digestive Diseases and Sciences·K U Peterson
Jan 1, 1984·The Journal of Membrane Biology·D J NelsonL G Palmer
May 1, 1988·The Journal of Membrane Biology·J S Stoddard, L Reuss
Jan 1, 1985·The Journal of Membrane Biology·D C ZeldinW M Armstrong
Jan 1, 1991·Comparative Biochemistry and Physiology. A, Comparative Physiology·J J RoslynC E Palant
Dec 1, 1992·Comparative Biochemistry and Physiology. Comparative Physiology·D Cremaschi, C Porta
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Jan 1, 1994·The Journal of General Physiology·T A HemingL Reuss
Jan 1, 1984·The Journal of General Physiology·S A Weinman, L Reuss
Jun 1, 1984·The Journal of General Physiology·L Reuss, J L Costantin
Mar 1, 1985·The Journal of General Physiology·L Reuss, K U Petersen
May 1, 1991·The Journal of General Physiology·C U Cotton, L Reuss
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Mar 17, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·A James MoserR J Bridges
Oct 13, 2000·American Journal of Physiology. Cell Physiology·P LyallV Lyall

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