Cyclic AMP induces apolipoprotein E binding activity and promotes cholesterol efflux from a macrophage cell line to apolipoprotein acceptors.

The Journal of Biological Chemistry
J D SmithA S Plump

Abstract

RAW 264 mouse macrophage cells were stably transfected with human apolipoprotein E (apoE) expression vectors. Clonal derivatives were characterized for expression of the human apoE2, apoE3, and apoE4 isoforms. An apoE4-expressing clonal cell line and a non-expressing clonal control cell line were loaded overnight with either [3H]cholesterol or [3H]choline. The cells were washed and incubated for 24 h in serum-free medium with or without the addition of 8-bromo-cyclic AMP (8-Br-cAMP). Only the apoE-secreting cells and only in the presence of 8-Br-cAMP released large amounts of labeled cholesterol or phosphatidylcholine into the medium. Mass analyses of cellular free and esterified cholesterol confirmed the results of the labeling studies; a decrease in cellular cholesterol content was observed in the 8-Br-cAMP-treated apoE-secreting cells, concurrent with an increase in cholesterol found in the medium. FPLC analysis of the medium demonstrated that 8-Br-cAMP treatment of the apoE-secreting cells led to an increased size fraction and amount of a peak of secreted cholesterol which comigrated with apoE. The 8-Br-cAMP-mediated increase in cholesterol efflux was also observed in non-apoE-secreting cells incubated with exogenous apoE o...Continue Reading

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J L GoldsteinM S Brown
Feb 28, 1986·Biochimica Et Biophysica Acta·J DeLamatreG H Rothblat
Jan 1, 1986·Methods in Enzymology·S C RallR W Mahley
Jan 1, 1986·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·T L InnerarityR W Mahley
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S K BasuJ L Goldstein
Oct 1, 1994·The Journal of Clinical Investigation·A J MendezJ F Oram
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y HuangG Assmann

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Citations

Apr 14, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Kirsty GreenowDipak P Ramji
Jun 11, 2010·Molecular and Cellular Biochemistry·Alisha D HowardJose L Soulages
Jul 21, 2007·Pharmaceutical Research·An-Qiang SunFrederick J Suchy
May 4, 2005·Protein Expression and Purification·Kevin PearsonW Sean Davidson
Jun 24, 1998·Biochimica Et Biophysica Acta·S Yokoyama
Jul 17, 1999·Atherosclerosis·O Stein, Y Stein
Sep 27, 2000·Biochimica Et Biophysica Acta·Y TakahashiJ D Smith
Jun 30, 2000·Current Opinion in Cell Biology·D Hoekstra, S C van IJzendoorn
Jul 24, 2002·Protein Expression and Purification·Stacey E PanagotopulosW Sean Davidson
Dec 9, 2000·Biochimica Et Biophysica Acta·J F Oram
Apr 27, 1999·Biochimica Et Biophysica Acta·S W SakrG H Rothblat
Nov 6, 2010·Assay and Drug Development Technologies·Jun ZhangJohn P Vanden Heuvel
Dec 29, 2000·Current Opinion in Cardiology·A von EckardsteinG Assmann
Jul 6, 2000·Current Opinion in Lipidology·J F Oram, A M Vaughan
Jul 6, 2000·Current Opinion in Lipidology·A Daugherty
May 14, 2005·Current Opinion in Lipidology·Shinji Yokoyama
Jan 1, 1997·Annual Review of Nutrition·D A BernlohrL J Banaszak
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·J C CortonA Stauber
Aug 9, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Dao-Quan PengJonathan D Smith
Mar 24, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Delphine EberléRobert L Raffai
Feb 22, 2011·Journal of Lipid Research·Priya MalikJonathan D Smith
Mar 2, 2013·PloS One·Youfeng YangGuangjie Cheng
Aug 4, 2009·Future Lipidology·Elena Posse de Chaves, Vasanthy Narayanaswami
Feb 1, 2002·Biochemical and Biophysical Research Communications·Olga SteinYechezkiel Stein
Aug 5, 2000·Biochemical and Biophysical Research Communications·K SchwartzD P Wade
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M W Freeman
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·A T RemaleyH B Brewer
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y Takahashi, J D Smith
Oct 2, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Iris LorenziLucia Rohrer
Feb 28, 2001·Expert Opinion on Investigational Drugs·J F Oram
Nov 19, 2013·The American Journal of Cardiology·Maen D Abou ZikiRonald G Crystal

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