Cyclic-AMP inhibits cell growth and negatively interacts with platelet membrane glycoprotein expression on the Dami human megakaryoblastic cell line

Journal of Cellular Physiology
D VittetC Chevillard

Abstract

Intracellular signaling processes by which hematopoietic growth factors regulate megakaryocytopoiesis remain incompletely understood. Cyclic AMP (cAMP) has been shown to be implicated in the regulation of growth and differentiation in various normal and malignant cell types. Since a few studies have suggested the possible involvement of the cAMP pathway as one of the intracellular mechanisms whereby megakaryocytopoiesis may be regulated, we investigated the functional effects of cAMP on the human megakaryoblastic Dami cell line. We observed that exposure of Dami cells to cAMP analogs or to agents elevating intracellular cAMP levels yielded dose-dependent cell growth inhibition. Cell cycle progression analysis of cells predominantly synchronized at the G1/S boundary by prior treatment with hydroxyurea revealed that cAMP transiently accumulated cells in the G2/M phase, then slowing down cell cycle. On the other hand, immunofluorescence and Northern blot analysis of megakaryocytic differentiation marker expression showed that probes we have used significantly inhibited GPIb expression. Moreover, although these agents used alone did not affect GPIIb/IIIa expression, they markedly reversed phorbol ester-induced GPIIb/IIIa expression...Continue Reading

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Citations

Feb 12, 1997·European Journal of Pharmacology·K ZurbonsenC Chevillard
Jan 10, 2012·The American Journal of the Medical Sciences·Waseem ToumaGanesh C Kudva
Jan 15, 2009·International Journal of Cancer. Journal International Du Cancer·Lena OevermannChristoph A Ritter
Jul 1, 1997·The Journal of Laboratory and Clinical Medicine·T A DaviesE R Simons
Feb 1, 1997·Baillière's Clinical Haematology·H Saito
Aug 1, 1997·Biochemical Pharmacology·K ZurbonsenC Chevillard
Apr 3, 2001·The Journal of Laboratory and Clinical Medicine·M SchattnerM A Lazzari

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