PMID: 9432022Jan 31, 1998Paper

Cyclic AMP phosphodiesterases in human lymphocytes

British Journal of Haematology
S B ShethR W Colman

Abstract

The function of lymphocytes, like platelets, has been shown to be inhibited by agents which increase intracellular cyclic AMP. Two high-affinity cAMP phosphodiesterases (PDEs), the cyclic GMP-inhibited cAMP phosphodiesterase, PDE3, and the cAMP-specific phosphodiesterase PDE4, are known to regulate cAMP concentration in haemopoietic cells by degrading cAMP to AMP. We characterized the relative contribution of the two PDEs to total lymphocyte PDE activity. We then determined which of the different gene products, PDE3A, typical of myocardium and platelets, or PDE3B, typical of adipocytes, were present in lymphocytes. The PDE3-specific inhibitor, milrinone, and the PDE4 inhibitor, rolipram, suppressed hydrolysis by 70% and 30% respectively, which indicated that both PDE4 and PDE3 were present, and that PDE3 was predominant. RT-PCR yields the expected size fragment for the primer pair PDE3B and not for PDE3A. The DNA sequence obtained had >95% identity with PDE3B. PDE3B appears to be the major cAMP PDE in lymphocytes. In contrast to human platelets, human lymphocytes appear to contain the PDE3B subtype. Since PDE3B in adipocytes is subject to hormonal regulation, lymphocytes may be similarly modulated. Understanding the role of cAM...Continue Reading

Citations

May 5, 2000·Biochemical Pharmacology·D M Essayan
Sep 16, 1998·Current Opinion in Chemical Biology·M J Perry, G A Higgs
Aug 6, 1999·Journal of Clinical Pharmacology·W A BriggsJ Burdick
Aug 4, 2005·The Journal of Thoracic and Cardiovascular Surgery·Yukio SatoYasunori Sohara
May 5, 2001·Expert Opinion on Pharmacotherapy·F Sánchez-Madrid, R González-Amaro
Apr 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·John A MeyersAdam Lerner
May 5, 2001·Expert Opinion on Pharmacotherapy·A Ballinger, G Smith
Dec 13, 2005·The Biochemical Journal·Adam Lerner, Paul M Epstein
May 4, 2021·Frontiers in Cell and Developmental Biology·Paul M EpsteinStefan Brocke
Jun 8, 2021·Frontiers in Cell and Developmental Biology·Morgane SimaoClotilde Randriamampita

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