PMID: 9636212Jun 24, 1998Paper

Cyclic AMP regulates potassium channel expression in C6 glioma by destabilizing Kv1.1 mRNA

Proceedings of the National Academy of Sciences of the United States of America
M L AllenB L Tempel

Abstract

The tissue distributions and physiological properties of a variety of cloned voltage-gated potassium channel genes have been characterized extensively, yet relatively little is known about the mechanisms controlling expression of these genes. Here, we report studies on the regulation of Kv1.1 expressed endogenously in the C6 glioma cell line. We demonstrate that elevation of intracellular cAMP leads to the accelerated degradation of Kv1.1 RNA. The cAMP-induced decrease in Kv1.1 RNA is followed by a decrease in Kv1. 1 protein and a decrease in the whole cell sustained K+ current amplitude. Dendrotoxin-I, a relatively specific blocker of Kv1.1, blocks 96% of the sustained K+ current in glioma cells, causing a shift in the resting membrane potential from -40 mV to -7 mV. These data suggest that expression of Kv1.1 contributes to setting the resting membrane potential in undifferentiated glioma cells. We therefore suggest that receptor-mediated elevation of cAMP reduces outward K+ current density by acting at the translational level to destabilize Kv1.1 RNA, an additional mechanism for regulating potassium channel gene expression.

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Citations

Sep 9, 2008·Pflügers Archiv : European journal of physiology·Zoltán Rusznák, Géza Szucs
Sep 1, 2005·Canadian Journal of Applied Physiology = Revue Canadienne De Physiologie Appliquée·Phillip GardinerBruno Cormery
Sep 3, 2002·American Journal of Respiratory and Critical Care Medicine·Rolf GöggelStefan Uhlig
Jul 15, 2009·Journal of Neuroscience Methods·Roeland BuckinxJean-Michel Rigo
Aug 17, 2004·Journal of Cardiovascular Pharmacology and Therapeutics·Sanjeev WassonMary L Dohrmann
Feb 3, 2004·The Journal of Comparative Neurology·Yong LuEdwin W Rubel
Jun 20, 2003·Molecular Pharmacology·Judith A EnyeartJohn J Enyeart
Jul 27, 2000·The Journal of Biological Chemistry·J A EnyeartJ J Enyeart
Jun 19, 2013·Cancer Research·Hélène Lallet-DaherDavid Bernard
Jun 11, 2009·Nano Letters·Rebecca L Orndorff, Sandra J Rosenthal

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