Cyclic changes in NMDA receptor activation in hippocampal CA1 neurons after ischemia

Neuroscience Research
K OguroN Kawai

Abstract

We studied N-methyl-D-aspartate (NMDA) receptor-mediated synaptic potentials in CA1 pyramidal neurons using hippocampal slices of gerbils after transient forebrain ischemia. In the presence of 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and bicuculline, stimulation of Schaffer collateral/commissural fibers induced field excitatory postsynaptic potentials (fEPSP) activated by NMDA receptors. We found that in many slices after ischemia, prolonged low-frequency stimulation (0.1-10 Hz) caused repeated depression and potentiation of the NMDA-mediated fEPSP. Changes in fEPSP amplitude were dependent on stimulus frequency and the cycle frequency ranged from 0.08 to 2.5 cycles/min. These cyclic changes were blocked by application of BAPTA-AM, a membrane-permeable Ca2+ chelator, but were little affected by application of verapamil or by lowering the Ca2+ in bathing solution. Intracellular recordings from CA1 neurons revealed that low-frequency stimulation caused periodic depolarizations of membrane potential accompanied by depression of the excitatory postsynaptic potentials. The cyclic changes of fEPSPs were blocked by inhibitors of protein kinase C (PKC) but were unaffected by inhibitors of Ca2+/calmodulin-dependent protein kinase II ...Continue Reading

References

Sep 1, 1992·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·P AndinéH Hagberg
Sep 1, 1992·Trends in Neurosciences·Y Ben-AriP Bregestovski
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D E Pellegrini-GiampietroW A Pulsinelli
May 1, 1992·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·T KirinoN Kawai
Jan 1, 1991·Neuroscience·R Schmidt-Kastner, T F Freund
Apr 15, 1991·Biochemical and Biophysical Research Communications·R F BrunsH Nakano
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G A de ErausquinG Brooker
Apr 1, 1989·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·B K Siesjö, F Bengtsson
Dec 1, 1988·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·P AndinéH Hagberg
Jan 30, 1987·Biochemical and Biophysical Research Communications·H KaseM Kaneko
Aug 1, 1987·Neuroscience·S M RothmanR E Hauhart
Feb 1, 1987·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J K DeshpandeT Wieloch
Oct 1, 1984·Journal of Neurochemistry·G A Dienel
May 1, 1982·Annals of Neurology·W A PulsinelliF Plum
Nov 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H Li, A M Buchan
Jun 1, 1994·Current Opinion in Neurobiology·K W RocheR L Huganir

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Citations

Dec 3, 2003·Pathophysiology : the Official Journal of the International Society for Pathophysiology·B Schaller, R Graf
Dec 19, 1998·Neuroscience Research·K Kataoka, H Yanase
Apr 5, 2005·The International Journal of Neuroscience·Recep SutcuNamik Delibas

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