PMID: 8588975Aug 1, 1995Paper

Cyclic GMP accumulation in normal and diabetic primary culture adult rat ventricular cardiomyocytes: a minor role for nitric oxide in phosphorylase activation

Cellular Signalling
J A Buczek-Thomas, T B Miller

Abstract

The potential role of nitric oxide in the diabetes-induced hypersensitive activation of glycogen phosphorylase by epinephrine was investigated in adult rat ventricular cardiomyocytes. Pretreatment of normal and diabetic-derived cells with 1 mM sodium nitroprusside significantly diminished the phosphorylase activation response by nearly 20% in both normal and diabetic myocytes but failed to alter the hypersensitivity of the diabetic cells. Nitroprusside increased cGMP levels in both normal and diabetic myocytes although the effect was more pronounced in the diabetic cells. Epinephrine did not alter cellular cGMP content and cGMP levels were consistently lower in diabetic myocytes when compared with normal myocytes. Preincubation of ventricular myocytes with the nitric oxide synthase inhibitor L-iminoethyl ornithine did not affect phosphorylase activation. These data indicate that nitric oxide plays a minor role in phosphorylase activation by epinephrine in rat cardiomyocytes and suggest that signal transduction via nitric oxide is not affected by the onset of diabetes.

References

Feb 1, 1979·Circulation Research·G I Drummond, D L Severson
Jul 1, 1979·Circulation·F R CobbC C Roe
Jan 1, 1992·Trends in Pharmacological Sciences·T McCall, P Vallance
Nov 13, 1992·European Journal of Pharmacology·R E KlabundeU Förstermann
Sep 4, 1992·Cell·C J Lowenstein, S H Snyder
Oct 30, 1992·Biochemical and Biophysical Research Communications·S L Archer, V Hampl
Nov 14, 1992·Molecular and Cellular Biochemistry·J A Buczek-ThomasT B Miller
Dec 1, 1992·The American Journal of Physiology·A J BradyJ B Warren
Mar 11, 1992·The American Journal of Physiology·J A Buczek-ThomasT B Miller
Nov 1, 1990·British Journal of Pharmacology·D D ReesS Moncada
Apr 1, 1991·Circulation Research·D C BodeL L Brunton
Jun 1, 1988·Diabetologia·S Lenzen, U Panten
May 11, 1987·The American Journal of Physiology·C D WollebenT B Miller
Oct 1, 1987·Diabetes/metabolism Reviews·E J Rayfield, K Ishimura
May 1, 1972·Analytical Biochemistry·D P GilboeF Q Nuttall
Feb 11, 1984·The American Journal of Physiology·T B Miller
Jan 1, 1980·Annual Review of Biochemistry·R J Fletterick, N B Madsen
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J L BalligandT Michel
Jan 26, 1993·Biochemical Pharmacology·B MayerK Schmidt
Aug 18, 1993·Biochimica Et Biophysica Acta·H H SchmidtU Walter
Jul 1, 1993·The American Journal of Physiology·A J BradyS E Harding
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K TsouP Greengard

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Citations

Apr 26, 1995·Molecular and Cellular Biochemistry·J A Buczek-Thomas, T B Miller

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