Cyclic Mechanical Stretch Up-regulates Hepatoma-Derived Growth Factor Expression in Cultured Rat Aortic Smooth Muscle Cells

Bioscience Reports
Ying-Hsien KaoCheng-I Cheng

Abstract

Hepatoma-derived growth factor (HDGF) is a potent mitogen for vascular smooth muscle cells (SMCs) during embryogenesis and injury repair of vessel walls. Whether mechanical stimuli modulate HDGF expression remains unknown. This study aimed at investigating whether cyclic mechanical stretch plays a regulatory role in HDGF expression and regenerative cytokine production in aortic SMCs. A SMC cell line was grown on a silicone-based elastomer chamber with extracellular matrix coatings (either type I collagen or fibronectin) and received cyclic and uni-axial mechanical stretches with 10% deformation at frequency 1 Hz. Morphological observation showed that fibronectin coating provided better cell adhesion and spreading and that consecutive 6 hours of cyclic mechanical stretch remarkably induced reorientation and realignment of SMCs. Western blotting detection demonstrated that continuous mechanical stimuli elicited up-regulation of HDGF and PCNA, a cell proliferative marker. Signal kinetic profiling study indicated that cyclic mechanical stretch induced signaling activity in RhoA/ROCK and PI3K/Akt cascades. Kinase inhibition study further showed that blockade of PI3K activity suppressed the stretch-induced TNF-a, whereas RhoA/ROCK in...Continue Reading

References

Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M KlagsbrunJ A Smith
Sep 23, 1997·Biochemical and Biophysical Research Communications·Y IzumotoH Nakamura
Sep 17, 1998·The Journal of Clinical Investigation·J A Oliver, Q Al-Awqati
Mar 11, 2000·The Journal of Clinical Investigation·A D EverettC A McNamara
Apr 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Mohammad SotoudehJohn Y-J Shyy
Oct 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Paul R StandleyAntonino Camaratta
Nov 1, 2002·American Journal of Physiology. Cell Physiology·Andrew J PutnamDavid J Mooney
Mar 26, 2003·American Journal of Respiratory Cell and Molecular Biology·Paul G SmithSubhenu Chauduri
Jan 31, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Allen D EverettAmy Tucker
Dec 14, 2004·The Journal of Biological Chemistry·Jiaxu WangChristopher A McCulloch
Feb 1, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Anna ZampetakiQingbo Xu
Jan 5, 2006·Genes & Development·Wei-Xing Zong, Craig B Thompson
Oct 13, 2006·Journal of Leukocyte Biology·Marco E Bianchi
Jun 19, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Bao-Wei WangKou-Gi Shyu
Aug 24, 2007·Journal of Vascular Research·Ming-Juan QuZong-Lai Jiang
Nov 11, 2008·Molecular and Cellular Biochemistry·Wei-Jun CaiWolfgang Schaper
Nov 17, 2009·Journal of Hepatology·Ying-Hsien KaoMing-Hong Tai
Dec 21, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Jian-Hong ZhuNicholas A Flavahan
Apr 15, 2011·BMC Cell Biology·Allen D EverettDavid L Brautigan
Apr 19, 2011·VASA. Zeitschrift für Gefässkrankheiten·B-C ZhangY-W Xu
May 4, 2011·Atherosclerosis·Marcella MaddalunoArmando Ialenti
Mar 13, 2012·Cardiovascular Research·Delphine Gomez, Gary K Owens
May 2, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Soon-Mi LimAndreea Trache
Nov 6, 2014·Journal of Cellular Biochemistry·Airu NiuYi-Ping Li

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