PMID: 7513349Jan 1, 1994Paper

Cyclic nucleotide-gated channels of rat olfactory receptor cells: divalent cations control the sensitivity to cAMP

The Journal of General Physiology
J W Lynch, B Lindemann

Abstract

cAMP-gated channels were studied in inside-out membrane patches excised from the apical cellular pole of isolated olfactory receptor cells of the rat. In the absence of divalent cations the dose-response curve of activation of patch current by cAMP had a KM of 4.0 microM at -50 mV and of 2.5 microM at +50 mV. However, addition of 0.2 or 0.5 mM Ca2+ shifted the KM of cAMP reversibly to the higher cAMP concentrations of 33 or 90 microM, respectively, at -50 mV. Among divalent cations, the relative potency for inducing cAMP affinity shifts was: Ca2+ > Sr2+ > Mn2+ > Ba2+ > Mg2+, of which Mg2+ (up to 3 mM) did not shift the KM at all. This potency sequence corresponds closely to that required for the activation of calmodulin. However, the Ca(2+)-sensitivity is lower than expected for a calmodulin-mediated action. Brief (60 s) transient exposure to 3 mM Mg2+, in the absence of other divalent cations, had a protective effect in that following washout of Mg2+, subsequent exposure to 0.2 mM Ca2+ no longer caused affinity shifts. This protection effect did not occur in intact cells and was probably a consequence of patch excision, possibly representing ablation of a regulatory protein from the channel cyclic nucleotide binding site. Thus...Continue Reading

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Citations

Aug 1, 1995·Current Opinion in Neurobiology·B W Ache, A Zhainazarov
Jun 1, 1995·Current Opinion in Neurobiology·A L Zimmerman
Jun 1, 1997·Neurochemistry International·J NoéH Breer
Jun 15, 2004·Nature Neuroscience·Jonathan BradleyStephan Frings
Oct 1, 1996·The European Journal of Neuroscience·N ThüraüfH Hatt
Mar 2, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tatiana I RebrikJuan I Korenbrot
Apr 25, 2006·FEBS Letters·Simone PifferiAnna Menini
Oct 31, 2001·The European Journal of Neuroscience·C H WetzelH Hatt
Feb 20, 1997·Nature·T Kurahashi, A Menini
May 1, 1996·Journal of Neurobiology·D RestrepoD Schild
Apr 30, 1998·Physiological Reviews·D Schild, D Restrepo
Jan 16, 2021·Cell and Tissue Research·Anna BoccaccioSimone Pifferi
Nov 9, 1995·Biochimica Et Biophysica Acta·E TareilusH Breer

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