PMID: 6167289Aug 17, 1981Paper

Cyclic nucleotide metabolism in rat colonic epithelial cells with different proliferative activities

Biochimica Et Biophysica Acta
P A Craven, F R Derubertis

Abstract

Cyclic nucleotide metabolism was examined in rat distal colonic epithelial cells with different proliferative activities. Lower crypt cells had DNA synthetic rates 7-10-fold higher than surface cells. Without a phosphodiesterase inhibitor proliferative cells had reduced basal cyclic AMP-, cyclic GMP-, and cyclic AMP-dependent protein kinase activity ratios, as well as blunted cyclic AMP responses to prostaglandin E2 and vasoactive intestinal peptide compared to superficial cells. In the presence of 3-isobutyl-1-methylxanthine, basal cyclic AMP and responses to prostaglandin E2 and vasoactive intestinal peptide of proliferative cells exceeded values in superficial cells. This correlated with higher membrane adenylate cyclase activity in the proliferative cells. By contrast, particulate and soluble guanylate cyclase activities of superficial cells were higher than in proliferative cells. The apparent high Km soluble and particulate cyclic AMP and cyclic GMP phosphodiesterase activities of proliferative cells were 4-7-fold higher than those in superficial cells. Moreover, the apparent low Km soluble activity was absent in superficial cells. Thus, an altered rate of nucleotide degradation may mediate reduced cyclic AMP and cyclic G...Continue Reading

Citations

May 18, 1984·European Journal of Pharmacology·N BoigeM Laburthe
Jan 1, 1985·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·J L Díaz-JuárezJ C Prieto
Jul 1, 1991·Gut·G J PostonP J Guillou
Oct 1, 1983·The Journal of Clinical Investigation·P A CravenF R DeRubertis
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May 1, 1993·The Journal of Clinical Investigation·P ValetC Denis
Jul 1, 1985·Metabolism: Clinical and Experimental·P A Craven, F R DeRubertis
Dec 1, 1995·Gastroenterology·T S GaginellaA Keshavarzian
Jan 1, 1983·Differentiation; Research in Biological Diversity·T A Brasitus, R S Keresztes
Apr 19, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·E MorelN M Griffiths
Mar 1, 1996·The American Journal of Physiology·K T WilsonE B Chang
Oct 20, 1994·Biochimica Et Biophysica Acta·H M AukemaR S Chapkin

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