Cyclic Peptides for Efficient Detection of Collagen

Chembiochem : a European Journal of Chemical Biology
Koh K TakitaTakaki Koide

Abstract

We report here a new class of collagen-binding peptides, cyclic collagen-mimetic peptides (cCMPs), that efficiently hybridize with the triple-helix-forming portions of collagen. cCMPs are composed of two parallel collagen-like (Xaa-Yaa-Gly)n strands with both termini tethered by covalent linkages. Enzyme-linked immunosorbent assays and western blotting analysis showed that cCMPs exhibit more potent affinity toward collagen than reported collagen-binding peptides and can specifically detect different collagen polypeptides in a mixture of proteins. Collagen secreted from cultured cells was detected by confocal microscopy with fluorescein-labeled cCMP. The cCMP is also shown to detect sensitively folding intermediates in the endoplasmic reticulum, something that was difficult to visualize with conventional collagen detectors. Molecular-dynamics simulations suggested that a cCMP forms a more stably hybridized product than its single-chain counterpart; this could explain why cCMP has higher affinity toward denatured collagen. These results indicate the usefulness of cCMPs as tools for detecting denatured collagen.

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Citations

Aug 30, 2019·Organic & Biomolecular Chemistry·Vladimir Kubyshkin
Jul 26, 2019·Organic & Biomolecular Chemistry·Koh K TakitaTakaki Koide
Mar 12, 2020·Biomacromolecules·Aubrey J EllisonRonald T Raines
Jul 14, 2021·Nature Chemical Biology·Matthew R AronoffHelma Wennemers
Jun 6, 2020·Journal of Proteome Research·Julian L KesslerS Michael Yu

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