Cyclic Stretch Negatively Regulates IL-1β Secretion Through the Inhibition of NLRP3 Inflammasome Activation by Attenuating the AMP Kinase Pathway

Frontiers in Physiology
Kentaro MaruyamaEiji Nemoto

Abstract

Macrophages are immune cells of hematopoietic origin that play diverse roles in host defenses and tissue homeostasis. In mechanical microenvironments, macrophages receive mechanical signals that regulate various cellular functions. However, the mechanisms by which mechanical signals influence the phenotype and function of macrophages in the process of inflammation have not yet been elucidated in detail. We herein examined the effects of cyclic stretch (CS) on NLR family, pyrin domain-containing 3 (NLRP3) inflammasome activation in J774.1, a murine macrophage cell line, and mouse primary bone marrow-derived macrophages. We showed that cyclic stretch inhibited adenosine triphosphate (ATP)-stimulated interleukin (IL)-1β secretion in lipopolysaccharide (LPS)-primed macrophages using ELISA and Western blot analyses. Cyclic stretch did not affect the degradation of the Inhibitor of κB or the nuclear translocation/transcriptional activity of nuclear factor (NF)-κB, suggesting that cyclic stretch-mediated inhibition was independent of the NF-κB signaling pathway. Consistent with these results, cyclic stretch did not affect the LPS-induced expression of inflammasome components, such as pro-IL-1β and NLRP3, which is known to require the ...Continue Reading

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Citations

Jan 16, 2019·Journal of Leukocyte Biology·Hemant Joshi, Sharon Celeste Morley
Oct 19, 2019·Tissue Engineering and Regenerative Medicine·Tae Heung KangMyung Chul Lee
Jan 29, 2021·The Journal of Pathology·Gwenda F VasseBarbro N Melgert
Apr 16, 2021·Frontiers in Cell and Developmental Biology·Joan-Carles EscolanoJochen Guck
Jun 29, 2021·Frontiers in Immunology·Yang LiQianzhou Jiang
Aug 6, 2021·Journal of Inflammation Research·Shafiu A Umar ShingeFengxu Yu
Oct 12, 2021·Frontiers in Immunology·Hamza AtchaWendy F Liu

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Methods Mentioned

BETA
ELISA
nuclear translocation
Assay
PCR
Enzyme-Linked Immunosorbent Assay

Software Mentioned

Softmax

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