Cyclic tensile stretch inhibition of nitric oxide release from osteoblast-like cells is both G protein and actin-dependent

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
F HaraC Hamanishi

Abstract

Recent reports indicate the alteration of nitric oxide (NO) synthesis with mechanical stress loaded on the osteoblast and NO is considered to have a significant role in mechanotransduction. We found the involvement of guanine-nucleotide-binding regulatory proteins (G proteins), especially Gi, in stress-inhibited NO release of osteoblast-like cells (JOR:17;593-597, 1999). To determine further the mechanism involved in this process, we measured c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) activity under cyclic tensile stretch loaded on osteoblast-like cells. Cyclic stretch significantly enhanced JNK/SAPK activity and pertussis toxin clearly reversed stress-enhanced JNK/SAPK activity. Cytochalasin D, actin microfilament disrupting reagent, also abolished the stress activation of JNK/SAPK. We propose a model for signaling events induced by cyclic tensile stretch, namely a transmembrane mechanosensor which couples Gi-protein, actin cytoskeleton and finally activates JNK/SAPK activity of osteoblasts.

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Citations

Jan 14, 2012·Cellular and Molecular Life Sciences : CMLS·Hayat MuhammadEfrat Monsonego Ornan
Dec 11, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Keyvan BehnamElsa J Brochmann
Sep 9, 2004·Science's STKE : Signal Transduction Knowledge Environment·Millie Hughes-Fulford
Mar 4, 2006·Biochemical and Biophysical Research Communications·Astrid LiedertAnita Ignatius
Dec 6, 2008·Biochemical and Biophysical Research Communications·Hongbin LuKwoksui Leung
Dec 16, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yeung-Jen ChenFeng-Sheng Wang
May 17, 2005·Calcified Tissue International·N YamamotoC Hamanishi
Aug 25, 2006·American Journal of Physiology. Cell Physiology·Pen-Hsiu Grace ChaoClark T Hung
Mar 8, 2002·The Journal of Biological Chemistry·Wanli WeiJohn W Kusiak
Jan 23, 2016·Journal of Tissue Engineering·Hye-Sun YuIvan Wall
Feb 21, 2019·Biomechanics and Modeling in Mechanobiology·Eoin McEvoyPatrick McGarry
Mar 16, 2016·Journal of Tissue Engineering·Hye-Sun YuIvan Wall

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