Cyclin B-cdk activity stimulates meiotic rereplication in budding yeast

Genetics
R StrichKatrina F Cooper

Abstract

Haploidization of gametes during meiosis requires a single round of premeiotic DNA replication (meiS) followed by two successive nuclear divisions. This study demonstrates that ectopic activation of cyclin B/cyclin-dependent kinase in budding yeast recruits up to 30% of meiotic cells to execute one to three additional rounds of meiS. Rereplication occurs prior to the meiotic nuclear divisions, indicating that this process is different from the postmeiotic mitoses observed in other fungi. The cells with overreplicated DNA produced asci containing up to 20 spores that were viable and haploid and demonstrated Mendelian marker segregation. Genetic tests indicated that these cells executed the meiosis I reductional division and possessed a spindle checkpoint. Finally, interfering with normal synaptonemal complex formation or recombination increased the efficiency of rereplication. These studies indicate that the block to rereplication is very different in meiotic and mitotic cells and suggest a negative role for the recombination machinery in allowing rereplication. Moreover, the production of haploids, regardless of the genome content, suggests that the cell counts replication cycles, not chromosomes, in determining the number of n...Continue Reading

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Citations

Jan 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kara E SawarynskiGeorge S Brush
Jan 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Randy Strich
Mar 15, 2005·Yeast
Dec 2, 2006·Biochimica Et Biophysica Acta·Kara E SawarynskiGeorge S Brush
Oct 3, 2014·PloS One·María-Dolores Rey, Pilar Prieto
May 11, 2006·The Journal of Biological Chemistry·Karen Schindler, Edward Winter

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