Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis.

The Journal of Cell Biology
Bernhard StraussJ Pines

Abstract

There is remarkable redundancy between the Cyclin-Cdk complexes that comprise the cell cycle machinery. None of the mammalian A-, D-, or E-type cyclins are required in development until implantation, and only Cdk1 is essential for early cell divisions. Cyclin B1 is essential for development, but whether it is required for cell division is contentious. Here, we used a novel imaging approach to analyze Cyclin B1-null embryos from fertilization onward. We show that Cyclin B1-/- embryos arrest in G2 phase after just two divisions. This is the earliest arrest of any Cyclin known and places Cyclin B1 with cdk1 as the essential regulators of the cell cycle. We reintroduced mutant proteins into this genetically null background to determine why Cyclin B1 is constantly exported from the nucleus. We found that Cyclin B1 must be exported from the nucleus for the cell to prevent premature entry to mitosis, and retaining Cyclin B1-Cdk1 at the plasma membrane precludes entry to mitosis.

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Citations

May 12, 2019·Biology of Reproduction·Jessica Y ChotinerP Jeremy Wang
Jun 19, 2019·Cell Cycle·Nora Bouftas, Katja Wassmann
May 19, 2019·The Journal of Biological Chemistry·Saratchandra Singh KhumukchamBramanandam Manavathi
Jul 28, 2019·Biology of Reproduction·Jian LiQing-Yuan Sun
May 1, 2020·The EMBO Journal·Nadia HégaratHelfrid Hochegger
Jul 10, 2019·Stem Cell Research & Therapy·Ildercílio Mota de Souza LimaRodrigo Alexandre Panepucci
Apr 3, 2020·The Journal of Cell Biology·Mark JackmanJonathon Pines
Jun 23, 2018·The Journal of Cell Biology·Matthew C JonesMartin J Humphries
Aug 12, 2018·The Journal of Cell Biology·Jian LiYi-Xun Liu
Oct 12, 2019·FEBS Letters·Adrijana Crncec, Helfrid Hochegger
Dec 31, 2020·Journal of Cellular Physiology·Shion OsanaRyoichi Nagatomi
Jan 10, 2021·Journal of Cell Science·Elizabeth ScotchmanPaul Nurse
Nov 19, 2020·Molecular Systems Biology·Daniel SchwabeNikolaus Rajewsky
Mar 12, 2021·Frontiers in Cell and Developmental Biology·Lei LuoKun Zhang
May 20, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jonas Muhr, Daniel W Hagey
Aug 28, 2021·International Journal of Molecular Sciences·Martin AngerMarketa Charousova

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FRET

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