Cyclin B3 is a mitotic cyclin that promotes the metaphase-anaphase transition

Current Biology : CB
Kai Yuan, Patrick H O'Farrell

Abstract

The timing mechanism for mitotic progression is still poorly understood. The spindle assembly checkpoint (SAC), whose reversal upon chromosome alignment is thought to time anaphase [1-3], is functional during the rapid mitotic cycles of the Drosophila embryo; but its genetic inactivation had no consequence on the timing of the early mitoses. Mitotic cyclins-Cyclin A, Cyclin B, and Cyclin B3-influence mitotic progression and are degraded in a stereotyped sequence [4-11]. RNAi knockdown of Cyclins A and B resulted in a Cyclin B3-only mitosis in which anaphase initiated prior to chromosome alignment. Furthermore, in such a Cyclin B3-only mitosis, colchicine-induced SAC activation failed to block Cyclin B3 destruction, chromosome decondensation, or nuclear membrane re-assembly. Injection of Cyclin B proteins restored the ability of SAC to prevent Cyclin B3 destruction. Thus, SAC function depends on particular cyclin types. Changing Cyclin B3 levels showed that it accelerated progress to anaphase, even in the absence of SAC function. The impact of Cyclin B3 on anaphase initiation appeared to decline with developmental progress. Our results show that different cyclin types affect anaphase timing differently in the early embryonic div...Continue Reading

References

May 4, 1990·Cell·C F Lehner, P H O'Farrell
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·C L RiederG Sluder
Apr 4, 2001·The Journal of Cell Biology·N den Elzen, J Pines
Mar 7, 2003·Current Biology : CB·Arnaud Echard, Patrick H O'Farrell
Jul 9, 2004·Developmental Cell·Patrick MeraldiPeter K Sorger
Apr 10, 2007·Nature Cell Biology·Eulalie BuffinRoger E Karess
Apr 12, 2007·Nature Reviews. Molecular Cell Biology·Andrea Musacchio, Edward D Salmon
Feb 23, 2008·Current Biology : CB·Mark L McCleland, Patrick H O'Farrell
May 13, 2008·Molecular Cell·Rob WolthuisJonathon Pines
Jun 4, 2008·Genes & Development·Rami Rahal, Angelika Amon
Mar 11, 2009·The Journal of Cell Biology·Mark L McClelandPatrick H O'Farrell
Dec 3, 2009·The Journal of Cell Biology·Zohra RahmaniRoger E Karess
Jan 19, 2010·Nature Cell Biology·Raquel A OliveiraKim Nasmyth
Aug 25, 2010·The Journal of Cell Biology·Barbara Di Fiore, Jonathon Pines
Aug 25, 2010·The Journal of Cell Biology·Wouter van ZonRob M F Wolthuis
Jun 11, 2011·Molecular Cell·Mardo KõivomägiMart Loog
Dec 24, 2011·Journal of Cell Science·Pablo Lara-GonzalezStephen S Taylor
Sep 12, 2012·The Journal of Cell Biology·Kai YuanPatrick H O'Farrell
May 28, 2013·Nature Cell Biology·Weiping Wang, Marc W Kirschner
Oct 8, 2014·The Journal of Cell Biology·Dan LuDavid O Morgan

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Citations

Oct 27, 2015·Cell Cycle·Yu ZhangShao-Chen Sun
Jun 7, 2016·Mechanisms of Development·Viviane VieiraHelena Araujo
May 10, 2016·ELife·Benjamin M Stormo, Donald T Fox
Dec 13, 2016·Biology·Jonathan M ScholeyIngrid Brust-Mascher
Mar 30, 2017·The Journal of Cell Biology·John S PoultonMark Peifer
Mar 14, 2018·Biology Open·Hanako Hayashi, Bungo Akiyoshi
Sep 23, 2016·G3 : Genes - Genomes - Genetics·Mohammed BourouhAndrew Swan
Nov 28, 2018·Molecular & Cellular Proteomics : MCP·Zijing ZhangMariana F Wolfner
May 12, 2019·Biology of Reproduction·Jessica Y ChotinerP Jeremy Wang
Jun 19, 2019·Cell Cycle·Nora Bouftas, Katja Wassmann
Feb 7, 2019·The Journal of Cell Biology·Mehmet E KarasuKatja Wassmann
Mar 9, 2019·Genetics·Edward T Kipreos, Sander van den Heuvel
Oct 13, 2018·The Journal of Cell Biology·Haytham MehsenVincent Archambault
Feb 17, 2019·The Journal of Cell Biology·Yufei LiWei Li
Nov 3, 2020·PLoS Genetics·Damien GarridoVincent Archambault
Mar 15, 2019·Molecular & Cellular Proteomics : MCP·Zijing ZhangMariana F Wolfner
Jul 3, 2021·Biology·Boyang LiuJörg Großhans
Oct 14, 2021·Seminars in Cell & Developmental Biology·Ojas Deshpande, Ivo A Telley

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